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1qs1

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[[Image:1qs1.jpg|left|200px]]
 
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{{Structure
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==CRYSTAL STRUCTURE OF VEGETATIVE INSECTICIDAL PROTEIN2 (VIP2)==
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|PDB= 1qs1 |SIZE=350|CAPTION= <scene name='initialview01'>1qs1</scene>, resolution 1.5&Aring;
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<StructureSection load='1qs1' size='340' side='right'caption='[[1qs1]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1qs1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QS1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QS1 FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(+)_ADP-ribosyltransferase NAD(+) ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.30 2.4.2.30] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qs1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qs1 OCA], [https://pdbe.org/1qs1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qs1 RCSB], [https://www.ebi.ac.uk/pdbsum/1qs1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qs1 ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qs1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qs1 OCA], [http://www.ebi.ac.uk/pdbsum/1qs1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qs1 RCSB]</span>
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[https://www.uniprot.org/uniprot/Q844J9_BACTU Q844J9_BACTU]
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''CRYSTAL STRUCTURE OF VEGETATIVE INSECTICIDAL PROTEIN2 (VIP2)'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qs/1qs1_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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A member of the Bacillus-produced vegetative insecticidal proteins (VIPs) possesses high specificity against the major insect pest, corn rootworms, and belongs to a class of binary toxins and regulators of biological pathways distinct from classical A-B toxins. The 1.5 A resolution crystal structure of the enzymatic ADP-ribosyltransferase component, VIP2, from Bacillus cereus reveals structurally homologous N- and C-terminal alpha/beta domains likely representing the entire class of binary toxins and implying evolutionary relationships between families of ADP-ribosylating toxins. The crystal structure of the kinetically trapped VIP2-NAD complex identifies the NAD binding cleft within the C-terminal enzymatic domain and provides a structural basis for understanding the targeting and catalysis of the medically and environmentally important binary toxins. These structures furthermore provide specific experimental results to help resolve paradoxes regarding the specific mechanism of ADP-ribosylation of actin by implicating ground state destabilization and nicotinamide product sequestration as the major driving forces for catalysis.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qs1 ConSurf].
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1QS1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QS1 OCA].
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<div style="clear:both"></div>
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__TOC__
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==Reference==
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</StructureSection>
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Evolution and mechanism from structures of an ADP-ribosylating toxin and NAD complex., Han S, Craig JA, Putnam CD, Carozzi NB, Tainer JA, Nat Struct Biol. 1999 Oct;6(10):932-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10504727 10504727]
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[[Category: Bacillus cereus]]
[[Category: Bacillus cereus]]
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[[Category: NAD(+) ADP-ribosyltransferase]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Carozzi NB]]
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[[Category: Carozzi, N B.]]
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[[Category: Craig JA]]
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[[Category: Craig, J A.]]
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[[Category: Han S]]
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[[Category: Han, S.]]
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[[Category: Putnam CD]]
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[[Category: Putnam, C D.]]
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[[Category: Tainer JA]]
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[[Category: Tainer, J A.]]
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[[Category: alpha-beta protein]]
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[[Category: binary toxin]]
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[[Category: insecticial protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:18:12 2008''
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Current revision

CRYSTAL STRUCTURE OF VEGETATIVE INSECTICIDAL PROTEIN2 (VIP2)

PDB ID 1qs1

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