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| | ==Structure of the Mus musclus Langerin carbohydrate recognition domain in complex with glucose== | | ==Structure of the Mus musclus Langerin carbohydrate recognition domain in complex with glucose== |
| - | <StructureSection load='5m62' size='340' side='right' caption='[[5m62]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='5m62' size='340' side='right'caption='[[5m62]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5m62]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M62 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5m62]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M62 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5k8y|5k8y]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m62 OCA], [http://pdbe.org/5m62 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m62 RCSB], [http://www.ebi.ac.uk/pdbsum/5m62 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m62 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m62 OCA], [https://pdbe.org/5m62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m62 RCSB], [https://www.ebi.ac.uk/pdbsum/5m62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m62 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CLC4K_MOUSE CLC4K_MOUSE]] Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells.<ref>PMID:11777972</ref> <ref>PMID:18322168</ref> | + | [https://www.uniprot.org/uniprot/CLC4K_MOUSE CLC4K_MOUSE] Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells.<ref>PMID:11777972</ref> <ref>PMID:18322168</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aretz, J]] | + | [[Category: Large Structures]] |
| - | [[Category: Loll, B]] | + | [[Category: Mus musculus]] |
| - | [[Category: Rademacher, C]] | + | [[Category: Aretz J]] |
| - | [[Category: Wahl, M C]] | + | [[Category: Loll B]] |
| - | [[Category: C-type lectin]] | + | [[Category: Rademacher C]] |
| - | [[Category: Calcium binding]] | + | [[Category: Wahl MC]] |
| - | [[Category: Carbohydrate binding protein]]
| + | |
| - | [[Category: Crd domain]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Lectin]]
| + | |
| Structural highlights
5m62 is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 1.7Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
CLC4K_MOUSE Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells.[1] [2]
Publication Abstract from PubMed
The recognition of pathogen surface polysaccharides by glycan-binding proteins is a cornerstone of innate host defense. Many members of the C-type lectin receptor family serve as pattern recognition receptors facilitating pathogen uptake, antigen processing, and immunomodulation. Despite the high evolutionary pressure in host-pathogen interactions, it is still widely assumed that genetic homology conveys similar specificities. Here, we investigate the ligand specificities of the human and murine forms of the myeloid C-type lectin receptor Langerin for simple and complex ligands augmented by structural insight on murine Langerin. Whereas the two homologs share the same three-dimensional structure and recognize simple ligands identically, a screening of more than 300 bacterial polysaccharides revealed highly diverging avidity and selectivity for larger and more complex glycans. Structural and evolutionary conservation analysis identified a highly variable surface adjacent to the canonic binding site potentially forming a secondary site of interaction for large glycans.
Bacterial polysaccharide specificity of the pattern recognition receptor Langerin is highly species dependent.,Hanske J, Schulze J, Aretz J, McBride R, Loll B, Schmidt H, Knirel Y, Rabsch W, Wahl MC, Paulson JC, Rademacher C J Biol Chem. 2016 Nov 30. pii: jbc.M116.751750. PMID:27903635[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Valladeau J, Clair-Moninot V, Dezutter-Dambuyant C, Pin JJ, Kissenpfennig A, Mattei MG, Ait-Yahia S, Bates EE, Malissen B, Koch F, Fossiez F, Romani N, Lebecque S, Saeland S. Identification of mouse langerin/CD207 in Langerhans cells and some dendritic cells of lymphoid tissues. J Immunol. 2002 Jan 15;168(2):782-92. PMID:11777972
- ↑ Idoyaga J, Cheong C, Suda K, Suda N, Kim JY, Lee H, Park CG, Steinman RM. Cutting edge: langerin/CD207 receptor on dendritic cells mediates efficient antigen presentation on MHC I and II products in vivo. J Immunol. 2008 Mar 15;180(6):3647-50. PMID:18322168
- ↑ Hanske J, Schulze J, Aretz J, McBride R, Loll B, Schmidt H, Knirel Y, Rabsch W, Wahl MC, Paulson JC, Rademacher C. Bacterial polysaccharide specificity of the pattern recognition receptor Langerin is highly species dependent. J Biol Chem. 2016 Nov 30. pii: jbc.M116.751750. PMID:27903635 doi:http://dx.doi.org/10.1074/jbc.M116.751750
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