1qsn

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:17, 14 February 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1qsn.gif|left|200px]]
 
-
{{Structure
+
==CRYSTAL STRUCTURE OF TETRAHYMENA GCN5 WITH BOUND COENZYME A AND HISTONE H3 PEPTIDE==
-
|PDB= 1qsn |SIZE=350|CAPTION= <scene name='initialview01'>1qsn</scene>, resolution 2.20&Aring;
+
<StructureSection load='1qsn' size='340' side='right'caption='[[1qsn]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=COA:COENZYME+A'>COA</scene>
+
<table><tr><td colspan='2'>[[1qsn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QSN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QSN FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qsn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qsn OCA], [https://pdbe.org/1qsn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qsn RCSB], [https://www.ebi.ac.uk/pdbsum/1qsn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qsn ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qsn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qsn OCA], [http://www.ebi.ac.uk/pdbsum/1qsn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qsn RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/Q27198_TETTH Q27198_TETTH]
-
 
+
== Evolutionary Conservation ==
-
'''CRYSTAL STRUCTURE OF TETRAHYMENA GCN5 WITH BOUND COENZYME A AND HISTONE H3 PEPTIDE'''
+
[[Image:Consurf_key_small.gif|200px|right]]
-
 
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==Overview==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qs/1qsn_consurf.spt"</scriptWhenChecked>
-
Gene activation is a highly regulated process that requires the coordinated action of proteins to relieve chromatin repression and to promote transcriptional activation. Nuclear histone acetyltransferase (HAT) enzymes provide a mechanistic link between chromatin destabilization and gene activation by acetylating the epsilon-amino group of specific lysine residues within the aminoterminal tails of core histones to facilitate access to DNA by transcriptional activators. Here we report the high-resolution crystal structure of the HAT domain of Tetrahymena GCN5 (tGCN5) bound with both its physiologically relevant ligands, coenzyme A (CoA) and a histone H3 peptide, and the structures of nascent tGCN5 and a tGCN5/acetyl-CoA complex. Our structural data reveal histone-binding specificity for a random-coil structure containing a G-K-X-P recognition sequence, and show that CoA is essential for reorienting the enzyme for histone binding. Catalysis appears to involve water-mediated proton extraction from the substrate lysine by a glutamic acid general base and a backbone amide that stabilizes the transition-state reaction intermediate. Comparison with related N-acetyltransferases indicates a conserved structural framework for CoA binding and catalysis, and structural variability in regions associated with substrate-specific binding.
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==About this Structure==
+
</jmolCheckbox>
-
1QSN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QSN OCA].
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qsn ConSurf].
-
 
+
<div style="clear:both"></div>
-
==Reference==
+
__TOC__
-
Structure of Tetrahymena GCN5 bound to coenzyme A and a histone H3 peptide., Rojas JR, Trievel RC, Zhou J, Mo Y, Li X, Berger SL, Allis CD, Marmorstein R, Nature. 1999 Sep 2;401(6748):93-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10485713 10485713]
+
</StructureSection>
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Tetrahymena thermophila]]
[[Category: Tetrahymena thermophila]]
-
[[Category: Allis, C David.]]
+
[[Category: Berger SL]]
-
[[Category: Berger, S L.]]
+
[[Category: David Allis C]]
-
[[Category: Li, X.]]
+
[[Category: Li X]]
-
[[Category: Marmorstein, R.]]
+
[[Category: Marmorstein R]]
-
[[Category: Mo, Y.]]
+
[[Category: Mo Y]]
-
[[Category: Rojas, J R.]]
+
[[Category: Rojas JR]]
-
[[Category: Trievel, R C.]]
+
[[Category: Trievel RC]]
-
[[Category: Zhou, J.]]
+
[[Category: Zhou J]]
-
[[Category: coa-binding protein]]
+
-
[[Category: gcn5-related n-acetyltransferase]]
+
-
[[Category: histone acetyltransferase]]
+
-
[[Category: ternary complex]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:18:26 2008''
+

Current revision

CRYSTAL STRUCTURE OF TETRAHYMENA GCN5 WITH BOUND COENZYME A AND HISTONE H3 PEPTIDE

PDB ID 1qsn

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qsn"
Personal tools