This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1qso

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:17, 14 February 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1qso.gif|left|200px]]
 
-
{{Structure
+
==Histone Acetyltransferase HPA2 from Saccharomyces Cerevisiae==
-
|PDB= 1qso |SIZE=350|CAPTION= <scene name='initialview01'>1qso</scene>, resolution 2.90&Aring;
+
<StructureSection load='1qso' size='340' side='right'caption='[[1qso]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[1qso]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QSO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QSO FirstGlance]. <br>
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Histone_acetyltransferase Histone acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.48 2.3.1.48] </span>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
-
|GENE=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qso OCA], [https://pdbe.org/1qso PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qso RCSB], [https://www.ebi.ac.uk/pdbsum/1qso PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qso ProSAT]</span></td></tr>
-
|DOMAIN=
+
</table>
-
|RELATEDENTRY=
+
== Function ==
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qso OCA], [http://www.ebi.ac.uk/pdbsum/1qso PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qso RCSB]</span>
+
[https://www.uniprot.org/uniprot/HPA2_YEAST HPA2_YEAST] In vitro, acetylates histone H3 'Lys-4' and 'Lys-14' and histone H4 'Lys-5' and 'Lys-12'.
-
}}
+
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qs/1qso_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qso ConSurf].
 +
<div style="clear:both"></div>
-
'''HISTONE ACETYLTRANSFERASE HPA2 FROM SACCHAROMYCES CEREVISIAE'''
+
==See Also==
-
 
+
*[[Histone acetyltransferase 3D structures|Histone acetyltransferase 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
We report the crystal structure of the yeast protein Hpa2 in complex with acetyl coenzyme A (AcCoA) at 2.4 A resolution and without cofactor at 2.9 A resolution. Hpa2 is a member of the Gcn5-related N-acetyltransferase (GNAT) superfamily, a family of enzymes with diverse substrates including histones, other proteins, arylalkylamines and aminoglycosides. In vitro, Hpa2 is able to acetylate specific lysine residues of histones H3 and H4 with a preference for Lys14 of histone H3. Hpa2 forms a stable dimer in solution and forms a tetramer upon binding AcCoA. The crystal structure reveals that the Hpa2 tetramer is stabilized by base-pair interactions between the adenine moieties of the bound AcCoA molecules. These base-pairs represent a novel method of stabilizing an oligomeric protein structure. Comparison of the structure of Hpa2 with those of other GNAT superfamily members illustrates a remarkably conserved fold of the catalytic domain of the GNAT family even though members of this family share low levels of sequence homology. This comparison has allowed us to better define the borders of the four sequence motifs that characterize the GNAT family, including a motif that is not discernable in histone acetyltransferases by sequence comparison alone. We discuss implications of the Hpa2 structure for the catalytic mechanism of the GNAT enzymes and the opportunity for multiple histone tail modification created by the tetrameric Hpa2 structure.
+
[[Category: Large Structures]]
-
 
+
-
==About this Structure==
+
-
1QSO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QSO OCA].
+
-
 
+
-
==Reference==
+
-
Crystal structure of the histone acetyltransferase Hpa2: A tetrameric member of the Gcn5-related N-acetyltransferase superfamily., Angus-Hill ML, Dutnall RN, Tafrov ST, Sternglanz R, Ramakrishnan V, J Mol Biol. 1999 Dec 17;294(5):1311-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10600387 10600387]
+
-
[[Category: Histone acetyltransferase]]
+
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Single protein]]
+
[[Category: Angus-Hill ML]]
-
[[Category: Angus-Hill, M L.]]
+
[[Category: Dutnall RN]]
-
[[Category: Dutnall, R N.]]
+
[[Category: Ramakrishnan V]]
-
[[Category: Ramakrishnan, V.]]
+
[[Category: Sterngalnz R]]
-
[[Category: Sterngalnz, R.]]
+
[[Category: Tafrov ST]]
-
[[Category: Tafrov, S T.]]
+
-
[[Category: histone acetyltransferase]]
+
-
[[Category: tetramer]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:18:29 2008''
+

Current revision

Histone Acetyltransferase HPA2 from Saccharomyces Cerevisiae

PDB ID 1qso

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qso"
Personal tools