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| - | [[Image:1qv8.gif|left|200px]] | |
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| - | {{Structure
| + | ==B-DNA Dodecamer d(CGCGAATTCGCG)2 complexed with proamine== |
| - | |PDB= 1qv8 |SIZE=350|CAPTION= <scene name='initialview01'>1qv8</scene>, resolution 2.5Å
| + | <StructureSection load='1qv8' size='340' side='right'caption='[[1qv8]], [[Resolution|resolution]] 2.50Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=BBZ:2'-(4-DIMETHYLAMINOPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5'-BI-BENZIMIDAZOLE'>BBZ</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> | + | <table><tr><td colspan='2'>[[1qv8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QV8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QV8 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BBZ:2-(4-DIMETHYLAMINOPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5-BI-BENZIMIDAZOLE'>BBZ</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qv8 OCA], [https://pdbe.org/1qv8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qv8 RCSB], [https://www.ebi.ac.uk/pdbsum/1qv8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qv8 ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=[[1qv4|1QV4]]
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qv8 OCA], [http://www.ebi.ac.uk/pdbsum/1qv8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qv8 RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''B-DNA Dodecamer d(CGCGAATTCGCG)2 complexed with proamine'''
| + | [[Category: Clark GR]] |
| - | | + | [[Category: Squire CJ]] |
| - | | + | |
| - | ==Overview==
| + | |
| - | New analogues of the minor groove binding ligand Hoechst 33342 have been investigated in an attempt to improve radioprotective activity. The synthesis, DNA binding, and in vitro radioprotective properties of methylproamine, the most potent derivative, are reported. Experiments with V79 cells have shown that methylproamine is approximately 100-fold more potent than the classical aminothiol radioprotector WR1065. The crystal structures of methylproamine and proamine complexes with the dodecamer d(CGCGAATTCGCG)(2) confirm that the new analogues also are minor groove binders. It is proposed that the DNA-bound methylproamine ligand acts as a reducing agent by an electron transfer mechanism, repairing transient radiation-induced oxidizing species on DNA.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1QV8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QV8 OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | In vitro studies with methylproamine: a potent new radioprotector., Martin RF, Broadhurst S, Reum ME, Squire CJ, Clark GR, Lobachevsky PN, White JM, Clark C, Sy D, Spotheim-Maurizot M, Kelly DP, Cancer Res. 2004 Feb 1;64(3):1067-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14871839 14871839]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Clark, G R.]] | + | |
| - | [[Category: Squire, C J.]] | + | |
| - | [[Category: b-dna]]
| + | |
| - | [[Category: minor groove binder]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:19:28 2008''
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