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| | ==Structure of tetrameric jacalin complexed with Gal alpha-(1,4) Gal== | | ==Structure of tetrameric jacalin complexed with Gal alpha-(1,4) Gal== |
| - | <StructureSection load='5j51' size='340' side='right' caption='[[5j51]], [[Resolution|resolution]] 1.67Å' scene=''> | + | <StructureSection load='5j51' size='340' side='right'caption='[[5j51]], [[Resolution|resolution]] 1.67Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5j51]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J51 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5j51]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J51 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.67Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ugw|1ugw]], [[1jac|1jac]], [[1kuj|1kuj]], [[1ws5|1ws5]], [[1ws4|1ws4]], [[1uh0|1uh0]], [[1m26|1m26]], [[1ugx|1ugx]], [[1uh1|1uh1]], [[1ugy|1ugy]], [[1ku8|1ku8]], [[4r6n|4r6n]], [[4r6o|4r6o]], [[4r6p|4r6p]], [[4r6q|4r6q]], [[4r6r|4r6r]], [[5j4x|5j4x]], [[5j50|5j50]], [[5j52|5j52]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j51 OCA], [http://pdbe.org/5j51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j51 RCSB], [http://www.ebi.ac.uk/pdbsum/5j51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j51 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j51 OCA], [https://pdbe.org/5j51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j51 RCSB], [https://www.ebi.ac.uk/pdbsum/5j51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j51 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LECA_ARTIN LECA_ARTIN]] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum. [[http://www.uniprot.org/uniprot/LECB3_ARTIN LECB3_ARTIN]] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum. | + | [https://www.uniprot.org/uniprot/LECA_ARTIN LECA_ARTIN] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5j51" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5j51" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Agglutinin 3D structures|Agglutinin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Artocarpus integer]] | | [[Category: Artocarpus integer]] |
| - | [[Category: Abhinav, K V]] | + | [[Category: Large Structures]] |
| - | [[Category: Sharma, K]] | + | [[Category: Abhinav KV]] |
| - | [[Category: Surolia, A]] | + | [[Category: Sharma K]] |
| - | [[Category: Vijayan, M]] | + | [[Category: Surolia A]] |
| - | [[Category: Beta-prism i fold]]
| + | [[Category: Vijayan M]] |
| - | [[Category: Galactose specific lectin]]
| + | |
| - | [[Category: Non-reducing sugar]]
| + | |
| - | [[Category: Plant lectin]]
| + | |
| - | [[Category: Post translational proteolysis]]
| + | |
| - | [[Category: Reducing]]
| + | |
| - | [[Category: Sugar binding protein]]
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| - | [[Category: T-antigen binding protein]]
| + | |
| Structural highlights
Function
LECA_ARTIN D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum.
Publication Abstract from PubMed
The crystal structures of jacalin complexed with Gal alpha-(1,4) Gal and Gal alpha-(1,3) Gal beta-(1,4) Gal have been determined with the primary objective of exploring the effect of linkage on the location of reducing and non-reducing sugars in the extended binding site of the lectin, an issue which has not been studied thoroughly. Contrary to the earlier surmise based on simple steric considerations, the two structures demonstrate that alpha-linked sugars can bind to jacalin with nonreducing sugar at the primary binding site. This is made possible substantially on account of the hitherto underestimated plasticity of a non-polar region of the extended binding site. Modeling studies involving conformational search and energy minimization, along with available crystallographic and thermodynamic data, indicate a strong preference for complexation with Gal beta-(1,3) Gal with the reducing Gal at the primary site, followed by that with Gal alpha-(1,3) Gal, with the reducing or non-reducing Gal located at the primary binding site. This observation is in consonance with the facility of jacalin to bind mucin type O-glycans containing T-antigen core. (c) 2016 IUBMB Life, 68(12):971-979, 2016.
Effect of linkage on the location of reducing and nonreducing sugars bound to jacalin.,Abhinav KV, Sharma K, Surolia A, Vijayan M IUBMB Life. 2016 Dec;68(12):971-979. doi: 10.1002/iub.1572. Epub 2016 Nov 3. PMID:27808459[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Abhinav KV, Sharma K, Surolia A, Vijayan M. Effect of linkage on the location of reducing and nonreducing sugars bound to jacalin. IUBMB Life. 2016 Dec;68(12):971-979. doi: 10.1002/iub.1572. Epub 2016 Nov 3. PMID:27808459 doi:http://dx.doi.org/10.1002/iub.1572
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