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1qwi

From Proteopedia

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[[Image:1qwi.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of E. coli OsmC==
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|PDB= 1qwi |SIZE=350|CAPTION= <scene name='initialview01'>1qwi</scene>, resolution 1.8&Aring;
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<StructureSection load='1qwi' size='340' side='right'caption='[[1qwi]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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<table><tr><td colspan='2'>[[1qwi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QWI FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE= OsmC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qwi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwi OCA], [https://pdbe.org/1qwi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qwi RCSB], [https://www.ebi.ac.uk/pdbsum/1qwi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qwi ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1n2f|1N2F]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qwi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwi OCA], [http://www.ebi.ac.uk/pdbsum/1qwi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qwi RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/OSMC_ECOLI OSMC_ECOLI] Preferentially metabolizes organic hydroperoxides over inorganic hydrogen peroxide.<ref>PMID:14627744</ref>
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== Evolutionary Conservation ==
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'''Crystal Structure of E. coli OsmC'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qw/1qwi_consurf.spt"</scriptWhenChecked>
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The osmotically inducible protein OsmC, like its better-characterized homolog, the organic hydroperoxide protein Ohr, is involved in defense against oxidative stress caused by exposure to organic hydroperoxides. The crystal structure of Escherichia coli OsmC reported here reveals that the protein is a tightly folded domain-swapped dimer with two active sites located at the monomer interface on opposite sides of the molecule. We demonstrate that OsmC preferentially metabolizes organic hydroperoxides over inorganic hydrogen peroxide. On the basis of structural and enzymatic similarities, we propose that the OsmC catalytic mechanism is analogous to that of the Ohr proteins and of the structurally unrelated peroxiredoxins, directly using highly reactive cysteine thiol groups to elicit hydroperoxide reduction.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1QWI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWI OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qwi ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Structural and functional features of the Escherichia coli hydroperoxide resistance protein OsmC., Lesniak J, Barton WA, Nikolov DB, Protein Sci. 2003 Dec;12(12):2838-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14627744 14627744]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Barton, W A.]]
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[[Category: Barton WA]]
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[[Category: Lesniak, J.]]
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[[Category: Lesniak J]]
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[[Category: Nikolov, D B.]]
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[[Category: Nikolov DB]]
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[[Category: hydroperoxide resistance]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:19:57 2008''
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Current revision

Crystal Structure of E. coli OsmC

PDB ID 1qwi

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