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5h4i

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==Unlinked NS2B-NS3 Protease from Zika Virus in complex with a compound fragment==
==Unlinked NS2B-NS3 Protease from Zika Virus in complex with a compound fragment==
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<StructureSection load='5h4i' size='340' side='right' caption='[[5h4i]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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<StructureSection load='5h4i' size='340' side='right'caption='[[5h4i]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5h4i]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H4I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H4I FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5h4i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Zika_virus Zika virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H4I FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7HQ:BENZIMIDAZOL-1-YLMETHANOL'>7HQ</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.004&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h4i OCA], [http://pdbe.org/5h4i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h4i RCSB], [http://www.ebi.ac.uk/pdbsum/5h4i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h4i ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7HQ:BENZIMIDAZOL-1-YLMETHANOL'>7HQ</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h4i OCA], [https://pdbe.org/5h4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h4i RCSB], [https://www.ebi.ac.uk/pdbsum/5h4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h4i ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/H8XX12_ZIKV H8XX12_ZIKV] Peptide 2k: Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.[SAAS:SAAS00882589]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Zika virus (ZIKV) has rapidly emerged as a global public health concern. Viral NS2B-NS3 protease processes viral polyprotein and is essential for the virus replication, making it an attractive antiviral drug target. We report crystal structures at 1.58-angstrom resolution of the unlinked NS2B-NS3 protease from ZIKV as free enzyme and bound to a peptide reversely oriented at the active site. The unlinked NS2B-NS3 protease adopts a closed conformation in which NS2B engages NS3 to form an empty substrate-binding site. A second protease in the same crystal binds to the residues K14K15G16E17 from the neighboring NS3 in reverse orientation, resisting proteolysis. These features of ZIKV NS2B-NS3 protease may accelerate the discovery of structure-based antiviral drugs against ZIKV and related pathogenic flaviviruses.
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Crystal structure of unlinked NS2B-NS3 protease from Zika virus.,Zhang Z, Li Y, Loh YR, Phoo WW, Hung AW, Kang C, Luo D Science. 2016 Dec 23;354(6319):1597-1600. doi: 10.1126/science.aai9309. Epub 2016, Dec 8. PMID:27940580<ref>PMID:27940580</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5h4i" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Li, Y]]
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[[Category: Large Structures]]
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[[Category: Zhang, Z Z]]
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[[Category: Zika virus]]
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[[Category: Non-structural protein 3]]
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[[Category: Li Y]]
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[[Category: Serine protease]]
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[[Category: Zhang ZZ]]
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[[Category: Viral protein]]
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[[Category: Zika virus protease]]
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Current revision

Unlinked NS2B-NS3 Protease from Zika Virus in complex with a compound fragment

PDB ID 5h4i

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