1qwy

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[[Image:1qwy.jpg|left|200px]]
 
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{{Structure
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==Latent LytM at 1.3 A resolution==
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|PDB= 1qwy |SIZE=350|CAPTION= <scene name='initialview01'>1qwy</scene>, resolution 1.30&Aring;
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<StructureSection load='1qwy' size='340' side='right'caption='[[1qwy]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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<table><tr><td colspan='2'>[[1qwy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QWY FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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|GENE= LytM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwy OCA], [https://pdbe.org/1qwy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qwy RCSB], [https://www.ebi.ac.uk/pdbsum/1qwy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qwy ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwy OCA], [http://www.ebi.ac.uk/pdbsum/1qwy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qwy RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/LYTM_STAA8 LYTM_STAA8] Peptidoglycan hydrolase (autolysin) specifically acting on polyglycine interpeptide bridges of the cell wall peptidoglycan.<ref>PMID:10220159</ref> <ref>PMID:8095258</ref>
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== Evolutionary Conservation ==
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'''Latent LytM at 1.3 A resolution'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qw/1qwy_consurf.spt"</scriptWhenChecked>
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LytM, an autolysin from Staphylococcus aureus, is a Zn(2+)-dependent glycyl-glycine endopeptidase with a characteristic HxH motif that belongs to the lysostaphin-type (MEROPS M23/37) of metallopeptidases. Here, we present the 1.3A crystal structure of LytM, the first structure of a lysostaphin-type peptidase. In the LytM structure, the Zn(2+) is tetrahedrally coordinated by the side-chains of N117, H210, D214 and H293, the second histidine of the HxH motif. Although close to the active-site, H291, the first histidine of the HxH motif, is not directly involved in Zn(2+)-coordination, and there is no water molecule in the coordination sphere of the Zn(2+), suggesting that the crystal structure shows a latent form of the enzyme. Although LytM has not previously been considered as a proenzyme, we show that a truncated version of LytM that lacks the N-terminal part with the poorly conserved Zn(2+) ligand N117 has much higher specific activity than full-length enzyme. This observation is consistent with the known removal of profragments in other lysostaphin-type proteins and with a prior observation of an active LytM degradation fragment in S.aureus supernatant. The "asparagine switch" in LytM is analogous to the "cysteine switch" in pro-matrix metalloproteases.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1QWY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWY OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qwy ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Latent LytM at 1.3A resolution., Odintsov SG, Sabala I, Marcyjaniak M, Bochtler M, J Mol Biol. 2004 Jan 16;335(3):775-85. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14687573 14687573]
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<references/>
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[[Category: Single protein]]
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__TOC__
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[[Category: Staphylococcus aureus]]
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</StructureSection>
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[[Category: Bochtler, M.]]
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[[Category: Large Structures]]
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[[Category: Marcyjaniak, M.]]
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[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
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[[Category: Odintsov, S G.]]
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[[Category: Bochtler M]]
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[[Category: Sabala, I.]]
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[[Category: Marcyjaniak M]]
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[[Category: lytm lysostaphin metalloprotease asparagine switch]]
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[[Category: Odintsov SG]]
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[[Category: Sabala I]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:20:08 2008''
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Current revision

Latent LytM at 1.3 A resolution

PDB ID 1qwy

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