1qx2

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[[Image:1qx2.gif|left|200px]]
 
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{{Structure
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==X-ray Structure of Calcium-loaded Calbindomodulin (A Calbindin D9k Re-engineered to Undergo a Conformational Opening) at 1.44 A Resolution==
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|PDB= 1qx2 |SIZE=350|CAPTION= <scene name='initialview01'>1qx2</scene>, resolution 1.44&Aring;
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<StructureSection load='1qx2' size='340' side='right'caption='[[1qx2]], [[Resolution|resolution]] 1.44&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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<table><tr><td colspan='2'>[[1qx2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QX2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QX2 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.44&#8491;</td></tr>
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|GENE= CALB3 OR S100D ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qx2 OCA], [https://pdbe.org/1qx2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qx2 RCSB], [https://www.ebi.ac.uk/pdbsum/1qx2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qx2 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qx2 OCA], [http://www.ebi.ac.uk/pdbsum/1qx2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qx2 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/S100G_BOVIN S100G_BOVIN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qx/1qx2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qx2 ConSurf].
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<div style="clear:both"></div>
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'''X-ray Structure of Calcium-loaded Calbindomodulin (A Calbindin D9k Re-engineered to Undergo a Conformational Opening) at 1.44 A Resolution'''
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==See Also==
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*[[S100 proteins 3D structures|S100 proteins 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The extent of conformational change that calcium binding induces in EF-hand proteins is a key biochemical property specifying Ca(2+) sensor versus signal modulator function. To understand how differences in amino acid sequence lead to differences in the response to Ca(2+) binding, comparative analyses of sequence and structures, combined with model building, were used to develop hypotheses about which amino acid residues control Ca(2+)-induced conformational changes. These results were used to generate a first design of calbindomodulin (CBM-1), a calbindin D(9k) re-engineered with 15 mutations to respond to Ca(2+) binding with a conformational change similar to that of calmodulin. The gene for CBM-1 was synthesized, and the protein was expressed and purified. Remarkably, this protein did not exhibit any non-native-like molten globule properties despite the large number of mutations and the nonconservative nature of some of them. Ca(2+)-induced changes in CD intensity and in the binding of the hydrophobic probe, ANS, implied that CBM-1 does undergo Ca(2+) sensorlike conformational changes. The X-ray crystal structure of Ca(2+)-CBM-1 determined at 1.44 A resolution reveals the anticipated increase in hydrophobic surface area relative to the wild-type protein. A nascent calmodulin-like hydrophobic docking surface was also found, though it is occluded by the inter-EF-hand loop. The results from this first calbindomodulin design are discussed in terms of progress toward understanding the relationships between amino acid sequence, protein structure, and protein function for EF-hand CaBPs, as well as the additional mutations for the next CBM design.
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==About this Structure==
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1QX2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QX2 OCA].
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==Reference==
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Designing sequence to control protein function in an EF-hand protein., Bunick CG, Nelson MR, Mangahas S, Hunter MJ, Sheehan JH, Mizoue LS, Bunick GJ, Chazin WJ, J Am Chem Soc. 2004 May 19;126(19):5990-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15137763 15137763]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bunick, C G.]]
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[[Category: Bunick CG]]
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[[Category: Bunick, G J.]]
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[[Category: Bunick GJ]]
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[[Category: Chazin, W J.]]
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[[Category: Chazin WJ]]
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[[Category: Mangahas, S.]]
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[[Category: Mangahas S]]
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[[Category: Mizoue, L S.]]
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[[Category: Mizoue LS]]
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[[Category: Nelson, M R.]]
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[[Category: Nelson MR]]
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[[Category: calbindin d9k]]
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[[Category: calcium-induced conformational response]]
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[[Category: calmodulin]]
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[[Category: ef-hand (helix-loop-helix) calcium binding protein]]
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[[Category: four-helix domain]]
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[[Category: protein engineering]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:20:14 2008''
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Current revision

X-ray Structure of Calcium-loaded Calbindomodulin (A Calbindin D9k Re-engineered to Undergo a Conformational Opening) at 1.44 A Resolution

PDB ID 1qx2

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