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| | ==Crystal structure of the Clostridium perfringens NetB toxin in the membrane inserted form== | | ==Crystal structure of the Clostridium perfringens NetB toxin in the membrane inserted form== |
| - | <StructureSection load='4h56' size='340' side='right' caption='[[4h56]], [[Resolution|resolution]] 3.90Å' scene=''> | + | <StructureSection load='4h56' size='340' side='right'caption='[[4h56]], [[Resolution|resolution]] 3.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4h56]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus perfringens" veillon and zuber 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H56 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H56 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4h56]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H56 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H56 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">netB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1502 "Bacillus perfringens" Veillon and Zuber 1898])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.9Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h56 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h56 OCA], [http://pdbe.org/4h56 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h56 RCSB], [http://www.ebi.ac.uk/pdbsum/4h56 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h56 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h56 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h56 OCA], [https://pdbe.org/4h56 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h56 RCSB], [https://www.ebi.ac.uk/pdbsum/4h56 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h56 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A8ULG6_CLOPF A8ULG6_CLOPF] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus perfringens veillon and zuber 1898]] | + | [[Category: Clostridium perfringens]] |
| - | [[Category: Basak, A K]] | + | [[Category: Large Structures]] |
| - | [[Category: Bokori-Brown, M]] | + | [[Category: Basak AK]] |
| - | [[Category: Cole, A R]] | + | [[Category: Bokori-Brown M]] |
| - | [[Category: Costa, S P.Fernandes da]] | + | [[Category: Cole AR]] |
| - | [[Category: Moss, D S]] | + | [[Category: Fernandes da Costa SP]] |
| - | [[Category: Naylor, C]] | + | [[Category: Moss DS]] |
| - | [[Category: Savva, C G]] | + | [[Category: Naylor C]] |
| - | [[Category: Titball, R W]] | + | [[Category: Savva CG]] |
| - | [[Category: Beta barrel]]
| + | [[Category: Titball RW]] |
| - | [[Category: Extracellular and membrane]]
| + | |
| - | [[Category: Necrotic enteritis]]
| + | |
| - | [[Category: Pore forming toxin]]
| + | |
| - | [[Category: Toxin]]
| + | |
| Structural highlights
Function
A8ULG6_CLOPF
Publication Abstract from PubMed
NetB is a pore-forming toxin produced by Clostridium perfringens and has been reported to play a major role in the pathogenesis of avian necrotic enteritis, a disease that has emerged due to the removal of antibiotics in animal feedstuffs. Here we present the crystal structure of the pore-form of NetB solved to 3.9A. The heptameric assembly shares structural homology to the Staphylococcal alpha-hemolysin. However, the rim domain, a region that is thought to interact with the target cell membrane shows sequence and structural divergence leading to the alteration of a phosphocholine binding pocket found in the staphylococcal toxins. Consistent with the structure we show that NetB does not bind phosphocholine efficiently but instead interacts directly with cholesterol leading to enhanced oligomerisation and pore formation. Finally we have identified conserved and non-conserved amino acid positions within the rim loops that significantly affect binding and toxicity of NetB. These findings present new insights into the mode of action of these pore-forming toxins enabling the design of more effective control measures against necrotic enteritis and providing potential new tools to the field of bionanotechnology.
Molecular architecture and functional analysis of NetB, a pore-forming toxin from Clostridium perfringens.,Savva CG, Fernandes da Costa SP, Bokori-Brown M, Naylor CE, Cole AR, Moss DS, Titball RW, Basak AK J Biol Chem. 2012 Dec 13. PMID:23239883[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Savva CG, Fernandes da Costa SP, Bokori-Brown M, Naylor CE, Cole AR, Moss DS, Titball RW, Basak AK. Molecular architecture and functional analysis of NetB, a pore-forming toxin from Clostridium perfringens. J Biol Chem. 2012 Dec 13. PMID:23239883 doi:http://dx.doi.org/10.1074/jbc.M112.430223
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