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| | ==Crystal structure of M. tuberculosis transcriptional regulator MOSR (Rv1049) in compex with DNA== | | ==Crystal structure of M. tuberculosis transcriptional regulator MOSR (Rv1049) in compex with DNA== |
| - | <StructureSection load='4fx4' size='340' side='right' caption='[[4fx4]], [[Resolution|resolution]] 3.10Å' scene=''> | + | <StructureSection load='4fx4' size='340' side='right'caption='[[4fx4]], [[Resolution|resolution]] 3.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4fx4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FX4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4fx4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FX4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FX4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1001Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fx0|4fx0]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fx4 OCA], [https://pdbe.org/4fx4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fx4 RCSB], [https://www.ebi.ac.uk/pdbsum/4fx4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fx4 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MT1079, Rv1049 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fx4 OCA], [http://pdbe.org/4fx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fx4 RCSB], [http://www.ebi.ac.uk/pdbsum/4fx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fx4 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/O53397_MYCTO O53397_MYCTO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Brugarolas, P]] | + | [[Category: Large Structures]] |
| - | [[Category: He, C]] | + | [[Category: Mycobacterium tuberculosis]] |
| - | [[Category: Dna binding]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Helix-turn-helix]] | + | [[Category: Brugarolas P]] |
| - | [[Category: Transcription regulator-dna complex]] | + | [[Category: He C]] |
| - | [[Category: Transcriptional repressor]]
| + | |
| Structural highlights
Function
O53397_MYCTO
Publication Abstract from PubMed
Mycobacterium tuberculosis thrives in oxidative environments such as the macrophage. In order to survive the bacterium must sense and adapt to the oxidative conditions. Several antioxidant defenses including a thick cell wall, millimolar concentrations of small molecule thiols and protective enzymes are known to help the bacterium withstand the oxidative stress. However, oxidation-sensing regulators that control these defenses have remained elusive. In this paper, we report a new oxidation-sensing regulator, Rv1049 or MosR (Mycobacterium tuberculosis oxidation-sensing Regulator). MosR is a transcriptional repressor of the MarR family, which, similarly to B. subtilis OhrR and S. aureus MgrA, dissociates from DNA in the presence of oxidants, enabling transcription. MosR senses oxidation through a pair of cysteines near the N-terminus (Cys10 and Cys12) that upon oxidation forms a disulfide bond. Disulfide formation rearranges a network of hydrogen bonds which leads to a large conformational change of the protein and dissociation from DNA. MosR has been shown previously to play an important role on survival of the bacterium in the macrophage. In this study we show that the main role of MosR is to upregulate expression of rv1050 (a putative exported oxidoreductase that has not yet been characterized) in response to oxidants and propose that it is through this role that MosR contributes to the bacterium survival in the macrophage.
The oxidation-sensing regulator (MosR) is a new redox dependent transcription factor in Mycobacterium tuberculosis.,Brugarolas P, Movahedzadeh F, Wang Y, Zhang N, Bartek IL, Gao YN, Voskuil MI, Franzblau SG, He C J Biol Chem. 2012 Sep 18. PMID:22992749[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Brugarolas P, Movahedzadeh F, Wang Y, Zhang N, Bartek IL, Gao YN, Voskuil MI, Franzblau SG, He C. The oxidation-sensing regulator (MosR) is a new redox dependent transcription factor in Mycobacterium tuberculosis. J Biol Chem. 2012 Sep 18. PMID:22992749 doi:http://dx.doi.org/10.1074/jbc.M112.388611
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