This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4g1j

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:24, 1 March 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==Sortase C1 of GBS Pilus Island 1==
==Sortase C1 of GBS Pilus Island 1==
-
<StructureSection load='4g1j' size='340' side='right' caption='[[4g1j]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
+
<StructureSection load='4g1j' size='340' side='right'caption='[[4g1j]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4g1j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_(serotype_v) Streptococcus agalactiae (serotype v)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G1J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G1J FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4g1j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae_serogroup_V Streptococcus agalactiae serogroup V]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G1J FirstGlance]. <br>
-
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4g1h|4g1h]]</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAG0647 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216466 Streptococcus agalactiae (serotype V)])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g1j OCA], [https://pdbe.org/4g1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g1j RCSB], [https://www.ebi.ac.uk/pdbsum/4g1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g1j ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g1j OCA], [http://pdbe.org/4g1j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g1j RCSB], [http://www.ebi.ac.uk/pdbsum/4g1j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g1j ProSAT]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/Q8E0S7_STRA5 Q8E0S7_STRA5]
-
Gram-positive bacteria assemble pili through class C sortase enzymes specialized in polymerizing pilin subunits into covalently linked, high-molecular-weight, elongated structures. Here we report the crystal structures of two class C sortases (SrtC1 and SrtC2) from Group B Streptococcus (GBS) Pilus Island 1. The structures show that both sortases are comprised of two domains: an 8-stranded beta-barrel catalytic core conserved among all sortase family members and a flexible N-terminal region made of two alpha-helices followed by a loop, known as the lid, which acts as a pseudo-substrate. In vitro experiments performed with recombinant SrtC enzymes lacking the N-terminal portion demonstrate that this region of the enzyme is dispensable for catalysis but may have key roles in substrate specificity and regulation. Moreover, in vitro FRET-based assays show that the LPXTG motif common to many sortase substrates is not the sole determinant of sortase C specificity during pilin protein recognition.
+
-
 
+
-
Structural basis for group B streptococcus pilus 1 sortases C regulation and specificity.,Cozzi R, Prigozhin D, Rosini R, Abate F, Bottomley MJ, Grandi G, Telford JL, Rinaudo CD, Maione D, Alber T PLoS One. 2012;7(11):e49048. doi: 10.1371/journal.pone.0049048. Epub 2012 Nov 8. PMID:23145064<ref>PMID:23145064</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 4g1j" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Alber, T]]
+
[[Category: Large Structures]]
-
[[Category: Cozzi, R]]
+
[[Category: Streptococcus agalactiae serogroup V]]
-
[[Category: Prigozhin, D M]]
+
[[Category: Alber T]]
-
[[Category: Cysteine protease]]
+
[[Category: Cozzi R]]
-
[[Category: Extracellular]]
+
[[Category: Prigozhin DM]]
-
[[Category: Transferase]]
+

Current revision

Sortase C1 of GBS Pilus Island 1

PDB ID 4g1j

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4g1j"
Personal tools