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| | ==Crystal structure of the MG2+ free VWA domain of plasmodium falciparum trap protein== | | ==Crystal structure of the MG2+ free VWA domain of plasmodium falciparum trap protein== |
| - | <StructureSection load='4f1k' size='340' side='right' caption='[[4f1k]], [[Resolution|resolution]] 1.87Å' scene=''> | + | <StructureSection load='4f1k' size='340' side='right'caption='[[4f1k]], [[Resolution|resolution]] 1.87Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4f1k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F1K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F1K FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4f1k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F1K FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4f1j|4f1j]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">THROMBOSPONDIN RELATED ANONYMOUS PROTEIN (TRAP), TRAP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 PLAFA])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f1k OCA], [https://pdbe.org/4f1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f1k RCSB], [https://www.ebi.ac.uk/pdbsum/4f1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f1k ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f1k OCA], [http://pdbe.org/4f1k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f1k RCSB], [http://www.ebi.ac.uk/pdbsum/4f1k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f1k ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Plafa]] | + | [[Category: Large Structures]] |
| - | [[Category: Kajander, T]] | + | [[Category: Plasmodium falciparum]] |
| - | [[Category: Knuuti, J]] | + | [[Category: Kajander T]] |
| - | [[Category: Permi, P]] | + | [[Category: Knuuti J]] |
| - | [[Category: Pihlajamaa, T]] | + | [[Category: Permi P]] |
| - | [[Category: Sharma, A]] | + | [[Category: Pihlajamaa T]] |
| - | [[Category: Cell adhesion]]
| + | [[Category: Sharma A]] |
| - | [[Category: Dinucleotide binding]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Rossmann fold]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Thrombospondin-related anonymous protein (TRAP), localized in the micronemes and on the surface of sporozoites of infamous malaria parasite Plasmodium, is a key molecule upon infection of mammalian host hepatocytes and invasion of mosquito salivary glands. TRAP contains two adhesive domains responsible for host cell recognition and invasion, and is known to be essential for infectivity. Here we report high resolution crystal structures of the A domain of Plasmodium falciparum TRAP with and without bound Mg2+. The structure reveals a von Willebrand factor A (vWA)-like fold and a functional metal ion-dependent adhesion site (MIDAS), as well as a potential heparan sulphate binding site. Site-directed mutagenesis and cell attachment assays were used to investigate the functional roles of discovered surface epitopes. The reported structures are the first determined for a complete vWA domain of parasitic origin, highlighting unique features among homologous domains from other proteins characterized hitherto. Some of these are conserved among Plasmodiae exclusively, while others may be common to apicomplexan organisms in general.
Structure of Plasmodium falciparum Thrombospondin-Related Anonymous Protein (TRAP) A domain highlights distinct features in apicomplexan von Willebrand Factor A homologues.,Pihlajamaa T, Kajander T, Knuuti J, Horkka K, Sharma A, Permi P Biochem J. 2013 Jan 15. PMID:23317521[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pihlajamaa T, Kajander T, Knuuti J, Horkka K, Sharma A, Permi P. Structure of Plasmodium falciparum Thrombospondin-Related Anonymous Protein (TRAP) A domain highlights distinct features in apicomplexan von Willebrand Factor A homologues. Biochem J. 2013 Jan 15. PMID:23317521 doi:10.1042/BJ20121058
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