5i55
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of the Virulent PSM-alpha3 Peptide Forming a Cross-alpha amyloid-like Fibril== | |
| + | <StructureSection load='5i55' size='340' side='right'caption='[[5i55]], [[Resolution|resolution]] 1.45Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5i55]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I55 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i55 OCA], [https://pdbe.org/5i55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i55 RCSB], [https://www.ebi.ac.uk/pdbsum/5i55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i55 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PSMA3_STAA8 PSMA3_STAA8] Peptide which can recruit, activate and subsequently lyse human neutrophils, thus eliminating the main cellular defense against infection. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Amyloids are ordered protein aggregates, found in all kingdoms of life, and are involved in aggregation diseases as well as in physiological activities. In microbes, functional amyloids are often key virulence determinants, yet the structural basis for their activity remains elusive. We determined the fibril structure and function of the highly toxic, 22-residue phenol-soluble modulin alpha3 (PSMalpha3) peptide secreted by Staphylococcus aureus PSMalpha3 formed elongated fibrils that shared the morphological and tinctorial characteristics of canonical cross-beta eukaryotic amyloids. However, the crystal structure of full-length PSMalpha3, solved de novo at 1.45 angstrom resolution, revealed a distinctive "cross-alpha" amyloid-like architecture, in which amphipathic alpha helices stacked perpendicular to the fibril axis into tight self-associating sheets. The cross-alpha fibrillation of PSMalpha3 facilitated cytotoxicity, suggesting that this assembly mode underlies function in S. aureus. | ||
| - | + | The cytotoxic Staphylococcus aureus PSMalpha3 reveals a cross-alpha amyloid-like fibril.,Tayeb-Fligelman E, Tabachnikov O, Moshe A, Goldshmidt-Tran O, Sawaya MR, Coquelle N, Colletier JP, Landau M Science. 2017 Feb 24;355(6327):831-833. doi: 10.1126/science.aaf4901. PMID:28232575<ref>PMID:28232575</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5i55" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Moshe | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| + | [[Category: Staphylococcus aureus]] | ||
| + | [[Category: Colletier J-P]] | ||
| + | [[Category: Coquelle N]] | ||
| + | [[Category: Landau M]] | ||
| + | [[Category: Moshe A]] | ||
| + | [[Category: Sawaya MR]] | ||
| + | [[Category: Tayeb-Fligelman E]] | ||
Current revision
Crystal Structure of the Virulent PSM-alpha3 Peptide Forming a Cross-alpha amyloid-like Fibril
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