5ict

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the Drosophila GluR1A ligand binding domain Y792T mutant complex with glutamate

Structural highlights

5ict is a 1 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.68Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GLR1_DROME Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists (By similarity). Forms ligand-gated ion channels which are activated by kainate.^[1]

Publication Abstract from PubMed

Phylogenetic analysis reveals AMPA, kainate, and NMDA receptor families in insect genomes, suggesting conserved functional properties corresponding to their vertebrate counterparts. However, heterologous expression of the Drosophila kainate receptor DKaiR1D and the AMPA receptor DGluR1A revealed novel ligand selectivity at odds with the classification used for vertebrate glutamate receptor ion channels (iGluRs). DKaiR1D forms a rapidly activating and desensitizing receptor that is inhibited by both NMDA and the NMDA receptor antagonist AP5; crystallization of the KaiR1D ligand-binding domain reveals that these ligands stabilize open cleft conformations, explaining their action as antagonists. Surprisingly, the AMPA receptor DGluR1A shows weak activation by its namesake agonist AMPA and also by quisqualate. Crystallization of the DGluR1A ligand-binding domain reveals amino acid exchanges that interfere with binding of these ligands. The unexpected ligand-binding profiles of insect iGluRs allows classical tools to be used in novel approaches for the study of synaptic regulation. VIDEO ABSTRACT.

Novel Functional Properties of Drosophila CNS Glutamate Receptors.,Li Y, Dharkar P, Han TH, Serpe M, Lee CH, Mayer ML Neuron. 2016 Dec 7;92(5):1036-1048. doi: 10.1016/j.neuron.2016.10.058. Epub 2016 , Nov 23. PMID:27889096^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ultsch A, Schuster CM, Laube B, Schloss P, Schmitt B, Betz H. Glutamate receptors of Drosophila melanogaster: cloning of a kainate-selective subunit expressed in the central nervous system. Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10484-8. PMID:1359540
↑ Li Y, Dharkar P, Han TH, Serpe M, Lee CH, Mayer ML. Novel Functional Properties of Drosophila CNS Glutamate Receptors. Neuron. 2016 Dec 7;92(5):1036-1048. doi: 10.1016/j.neuron.2016.10.058. Epub 2016 , Nov 23. PMID:27889096 doi:http://dx.doi.org/10.1016/j.neuron.2016.10.058

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ict"

Categories: Drosophila melanogaster | Large Structures | Dharkar P | Mayer ML

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Drosophila GluR1A ligand binding domain Y792T mutant complex with glutamate==
-<StructureSection load='5ict' size='340' side='right' caption='[[5ict]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
+<StructureSection load='5ict' size='340' side='right'caption='[[5ict]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ict]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ICT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ICT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ict]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ICT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ICT FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ict FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ict OCA], [http://pdbe.org/5ict PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ict RCSB], [http://www.ebi.ac.uk/pdbsum/5ict PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ict ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ict FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ict OCA], [https://pdbe.org/5ict PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ict RCSB], [https://www.ebi.ac.uk/pdbsum/5ict PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ict ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GLR1_DROME GLR1_DROME]] Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists (By similarity). Forms ligand-gated ion channels which are activated by kainate.<ref>PMID:1359540</ref>
+[https://www.uniprot.org/uniprot/GLR1_DROME GLR1_DROME] Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists (By similarity). Forms ligand-gated ion channels which are activated by kainate.<ref>PMID:1359540</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 5ict" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Glutamate receptor 3D structures|Glutamate receptor 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Dharkar, P]]
+[[Category: Drosophila melanogaster]]
-[[Category: Mayer, M L]]
+[[Category: Large Structures]]
-[[Category: Membrane protein]]
+[[Category: Dharkar P]]
+[[Category: Mayer ML]]

5ict

From Proteopedia

Current revision

Crystal structure of the Drosophila GluR1A ligand binding domain Y792T mutant complex with glutamate

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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