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1r3g

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[[Image:1r3g.gif|left|200px]]
 
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{{Structure
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==1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues==
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|PDB= 1r3g |SIZE=350|CAPTION= <scene name='initialview01'>1r3g</scene>, resolution 1.16&Aring;
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<StructureSection load='1r3g' size='340' side='right'caption='[[1r3g]], [[Resolution|resolution]] 1.16&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=GMU:2&#39;-O-[(2-GUANIDINIUM)ETHYL]-5-METHYLURIDINE+5&#39;-MONOPHOSPHATE'>GMU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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<table><tr><td colspan='2'>[[1r3g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R3G FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.16&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GMU:2-O-[(2-GUANIDINIUM)ETHYL]-5-METHYLURIDINE+5-MONOPHOSPHATE'>GMU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r3g OCA], [https://pdbe.org/1r3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r3g RCSB], [https://www.ebi.ac.uk/pdbsum/1r3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r3g ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r3g OCA], [http://www.ebi.ac.uk/pdbsum/1r3g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r3g RCSB]</span>
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__TOC__
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}}
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</StructureSection>
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[[Category: Large Structures]]
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'''1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues'''
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[[Category: Egli M]]
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[[Category: Lesnik E]]
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[[Category: Manoharan M]]
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==Overview==
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[[Category: Prakash TP]]
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[structure: see text] Oligonucleotides with a novel 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modification have been synthesized using a novel protecting group strategy for the guanidinium group. This modification enhances the binding affinity of oligonucleotides to RNA as well as duplex DNA (DeltaT(m) 3.2 degrees C per modification). The 2'-O-GE modified oligonucleotides exhibited exceptional resistance to nuclease degradation. The crystal structure of a palindromic duplex formed by a DNA oligonucleotide with a single 2'-O-GE modification was solved at 1.16 A resolution.
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[[Category: Puschl A]]
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[[Category: Tereshko V]]
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==About this Structure==
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1R3G is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R3G OCA].
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==Reference==
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2'-O-[2-(guanidinium)ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures., Prakash TP, Puschl A, Lesnik E, Mohan V, Tereshko V, Egli M, Manoharan M, Org Lett. 2004 Jun 10;6(12):1971-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15176796 15176796]
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[[Category: Protein complex]]
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[[Category: Egli, M.]]
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[[Category: Lesnik, E.]]
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[[Category: Manoharan, M.]]
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[[Category: Prakash, T P.]]
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[[Category: Puschl, A.]]
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[[Category: Tereshko, V.]]
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[[Category: dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:22:41 2008''
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Current revision

1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues

PDB ID 1r3g

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