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| - | [[Image:1r41.gif|left|200px]] | |
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| - | {{Structure
| + | ==Crystal structures of d(Gm5CGm5CGCGC) and d(GCGCGm5CGm5C): Effects of methylation on alternating DNA octamers== |
| - | |PDB= 1r41 |SIZE=350|CAPTION= <scene name='initialview01'>1r41</scene>, resolution 1.9Å
| + | <StructureSection load='1r41' size='340' side='right'caption='[[1r41]], [[Resolution|resolution]] 1.90Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=5CM:5-METHYL-2'-DEOXY-CYTIDINE-5'-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene> | + | <table><tr><td colspan='2'>[[1r41]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R41 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R41 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r41 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r41 OCA], [https://pdbe.org/1r41 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r41 RCSB], [https://www.ebi.ac.uk/pdbsum/1r41 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r41 ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=[[1r3z|1R3Z]]
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r41 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r41 OCA], [http://www.ebi.ac.uk/pdbsum/1r41 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r41 RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''Crystal structures of d(Gm5CGm5CGCGC) and d(GCGCGm5CGm5C): Effects of methylation on alternating DNA octamers'''
| + | [[Category: Pan B]] |
| - | | + | [[Category: Shi K]] |
| - | | + | [[Category: Sundaralingam M]] |
| - | ==Overview==
| + | [[Category: Tippin D]] |
| - | The crystal structures of two alternating octamers d(Gm(5)CGm(5)CGCGC) and d(GCGCGm(5)CGm(5)C) have been determined at 1.7 and 1.9 A resolution with R(work)/R(free) of 16.9/19.6% and 17.9/19.8%, respectively. The two octamers crystallized in the same tetragonal space group P4(3)2(1)2, with unit-cell parameters a = b = 43.0, c = 24.9 A and a = b = 43.1, c = 24.7 A, respectively. Both structures adopt the A-type double helix and the abutting crystal packing. Despite the different positions of the methylated cytosine in the sequence, the two octamers display essentially the same conformation. The close similarity of the two structures indicates that methylation at different positions in a DNA sequence does not affect the helical conformation of the alternating DNA octamer. Investigation of other related crystal structures implies that methylation of cytosine stabilizes the A-type conformation for alternating DNA sequences beginning with a 5'-purine but is beneficial to the Z-type conformation for alternating DNA sequences beginning with a 5'-pyrimidine.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1R41 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R41 OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Structures of d(Gm5)CGm5CGCGC) and d(GCGCGm5CGm5C): effects of methylation on alternating DNA octamers., Shi K, Pan B, Tippin D, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):61-5. Epub 2003 Dec, 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14684893 14684893]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Pan, B.]] | + | |
| - | [[Category: Shi, K.]] | + | |
| - | [[Category: Sundaralingam, M.]] | + | |
| - | [[Category: Tippin, D.]] | + | |
| - | [[Category: dna]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:22:53 2008''
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