5mpq
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5mpq is ON HOLD Authors: Williams, A.H. Description: Bulgecin A: The key to a broad-spectrum inhibitor that targets lytic transglycosylases [[Categ...) |
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| - | '''Unreleased structure''' | ||
| - | The | + | ==Bulgecin A: The key to a broad-spectrum inhibitor that targets lytic transglycosylases== |
| + | <StructureSection load='5mpq' size='340' side='right'caption='[[5mpq]], [[Resolution|resolution]] 1.78Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5mpq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis Neisseria meningitidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MPQ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLG:4-O-(4-O-SULFONYL-N-ACETYLGLUCOSAMININYL)-5-METHYLHYDROXY-L-PROLINE-TAURINE'>BLG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mpq OCA], [https://pdbe.org/5mpq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mpq RCSB], [https://www.ebi.ac.uk/pdbsum/5mpq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mpq ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9JXP1_NEIMB Q9JXP1_NEIMB] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Lytic transglycosylases (Lts) are involved in recycling, cell division, and metabolism of the peptidoglycan. They have been understudied for their usefulness as potential antibacterial targets due to their high redundancy in Gram-negative bacteria. Bulgecin A is an O-sulphonated glycopeptide that targets primarily soluble lytic tranglycosylases (Slt). It has been shown that bulgecin A increases the efficacy of beta-lactams that target penicillin bindings proteins (PBPs). Here, we present the high-resolution crystal structure of LtgA from Neisseria meningitidis strain MC58, a membrane bound homolog of Escherichia coli Slt, in complex with bulgecin A. The LtgA-bulgecin A complex reveals the mechanism of inhibition by bulgecin A at near atomic resolution. We further demonstrate that bulgecin A is not only a potent inhibitor of LtgA, but most importantly, it restores the efficacy of beta-lactam antibiotics in strains of N. meningitidis and Neisseria gonorrhoeae that have reduced susceptibility to beta-lactams. This is particularly relevant for N. gonorrhoeae where no vaccines are available. This work illustrates how best to target dangerous pathogens using a multiple drug target approach, a new and alternative approach to fighting antibiotic resistance. | ||
| - | + | Bulgecin A: The Key to a Broad-Spectrum Inhibitor That Targets Lytic Transglycosylases.,Williams AH, Wheeler R, Thiriau C, Haouz A, Taha MK, Boneca IG Antibiotics (Basel). 2017 Feb 22;6(1). pii: E8. doi: 10.3390/antibiotics6010008. PMID:28241458<ref>PMID:28241458</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Williams | + | <div class="pdbe-citations 5mpq" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Neisseria meningitidis]] | ||
| + | [[Category: Williams AH]] | ||
Current revision
Bulgecin A: The key to a broad-spectrum inhibitor that targets lytic transglycosylases
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