5mq8
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5mq8 is ON HOLD Authors: Piton, J., Gilet, L., Pellegrini, O., Leroy, M., Figaro, S., Condon, C. Description: Crystal structure of Rae1 (YacP) from...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Rae1 (YacP) from Bacillus subtilis== | |
| + | <StructureSection load='5mq8' size='340' side='right'caption='[[5mq8]], [[Resolution|resolution]] 2.25Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5mq8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MQ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MQ8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mq8 OCA], [https://pdbe.org/5mq8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mq8 RCSB], [https://www.ebi.ac.uk/pdbsum/5mq8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mq8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/YACP_BACSU YACP_BACSU] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The PIN domain plays a central role in cellular RNA biology and is involved in processes as diverse as rRNA maturation, mRNA decay and telomerase function. Here, we solve the crystal structure of the Rae1 (YacP) protein of Bacillus subtilis, a founding member of the NYN (Nedd4-BP1/YacP nuclease) subfamily of PIN domain proteins, and identify potential substrates in vivo Unexpectedly, degradation of a characterised target mRNA was completely dependent on both its translation and reading frame. We provide evidence that Rae1 associates with the B. subtilis ribosome and cleaves between specific codons of this mRNA in vivo Critically, we also demonstrate translation-dependent Rae1 cleavage of this substrate in a purified translation assay in vitro Multiple lines of evidence converge to suggest that Rae1 is an A-site endoribonuclease. We present a docking model of Rae1 bound to the B. subtilis ribosomal A-site that is consistent with this hypothesis and show that Rae1 cleaves optimally immediately upstream of a lysine codon (AAA or AAG) in vivo. | ||
| - | + | Rae1/YacP, a new endoribonuclease involved in ribosome-dependent mRNA decay in Bacillus subtilis.,Leroy M, Piton J, Gilet L, Pellegrini O, Proux C, Coppee JY, Figaro S, Condon C EMBO J. 2017 Mar 31. pii: e201796540. doi: 10.15252/embj.201796540. PMID:28363943<ref>PMID:28363943</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5mq8" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Bacillus subtilis subsp. subtilis str. 168]] |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Condon C]] | ||
| + | [[Category: Figaro S]] | ||
| + | [[Category: Gilet L]] | ||
| + | [[Category: Leroy M]] | ||
| + | [[Category: Pellegrini O]] | ||
| + | [[Category: Piton J]] | ||
Current revision
Crystal structure of Rae1 (YacP) from Bacillus subtilis
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