1r9p

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[[Image:1r9p.gif|left|200px]]
 
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{{Structure
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==Solution NMR Structure Of The Haemophilus Influenzae Iron-Sulfur Cluster Assembly Protein U (IscU) with Zinc Bound at the Active Site. Northeast Structural Genomics Consortium Target IR24.==
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|PDB= 1r9p |SIZE=350|CAPTION= <scene name='initialview01'>1r9p</scene>
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<StructureSection load='1r9p' size='340' side='right'caption='[[1r9p]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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<table><tr><td colspan='2'>[[1r9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R9P FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE= HI0377 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK11325 PRK11325]</span>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9p OCA], [https://pdbe.org/1r9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r9p RCSB], [https://www.ebi.ac.uk/pdbsum/1r9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r9p ProSAT], [https://www.topsan.org/Proteins/NESGC/1r9p TOPSAN]</span></td></tr>
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|RELATEDENTRY=[[1q48|1Q48]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9p OCA], [http://www.ebi.ac.uk/pdbsum/1r9p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r9p RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/ISCU_HAEIN ISCU_HAEIN] A scaffold on which IscS assembles Fe-S clusters. Subsequently gives the nascent cluster to other proteins. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters (By similarity).
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== Evolutionary Conservation ==
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'''Solution NMR Structure Of The Haemophilus Influenzae Iron-Sulfur Cluster Assembly Protein U (IscU) with Zinc Bound at the Active Site. Northeast Structural Genomics Consortium Target IR24.'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r9/1r9p_consurf.spt"</scriptWhenChecked>
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IscU is a highly conserved protein that serves as the scaffold for IscS-mediated assembly of iron-sulfur ([Fe-S]) clusters. We report the NMR solution structure of monomeric Haemophilus influenzae IscU with zinc bound at the [Fe-S] cluster assembly site. The compact core of the globular structure has an alpha-beta sandwich architecture with a three-stranded antiparallel beta-sheet and four alpha-helices. A nascent helix is located N-terminal to the core structure. The zinc is ligated by three cysteine residues and one histidine residue that are located in and near conformationally dynamic loops at one end of the IscU structure. Removal of the zinc metal by chelation results in widespread loss of structure in the apo form. The zinc-bound IscU may be a good model for iron-loaded IscU and may demonstrate structural features found in the [Fe-S] cluster bound form. Structural and functional similarities, genomic context in operons containing other homologous genes, and distributions of conserved surface residues support the hypothesis that IscU protein domains are homologous (i.e. derived from a common ancestor) with the SufE/YgdK family of [Fe-S] cluster assembly proteins.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1R9P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9P OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r9p ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Solution NMR structure of the iron-sulfur cluster assembly protein U (IscU) with zinc bound at the active site., Ramelot TA, Cort JR, Goldsmith-Fischman S, Kornhaber GJ, Xiao R, Shastry R, Acton TB, Honig B, Montelione GT, Kennedy MA, J Mol Biol. 2004 Nov 19;344(2):567-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15522305 15522305]
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</StructureSection>
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Acton, T B.]]
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[[Category: Acton TB]]
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[[Category: Cort, J R.]]
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[[Category: Cort JR]]
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[[Category: Kennedy, M A.]]
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[[Category: Kennedy MA]]
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[[Category: Montelione, G T.]]
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[[Category: Montelione GT]]
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[[Category: NESG, Northeast Structural Genomics Consortium.]]
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[[Category: Ramelot TA]]
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[[Category: Ramelot, T A.]]
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[[Category: Shastry R]]
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[[Category: Shastry, R.]]
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[[Category: Xiao R]]
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[[Category: Xiao, R.]]
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[[Category: 3beta strand]]
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[[Category: 4 alpha helix]]
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[[Category: iron-sulfur cluster binding]]
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[[Category: nesg]]
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[[Category: northeast structural genomics consortium]]
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[[Category: protein structure initiative]]
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[[Category: psi-2]]
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[[Category: structural genomic]]
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[[Category: three conserved cy]]
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[[Category: zinc binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:25:04 2008''
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Current revision

Solution NMR Structure Of The Haemophilus Influenzae Iron-Sulfur Cluster Assembly Protein U (IscU) with Zinc Bound at the Active Site. Northeast Structural Genomics Consortium Target IR24.

PDB ID 1r9p

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