DXP reductoisomerase

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<StructureSection load='3zhy' size='350' side='right' caption='DXP reductoisomerase complex with NADPH, Mn+2 ion and anti-malaria drug fosmidomycin (PDB code [[3zhy]])' scene='70/708083/Cv/1'>
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<StructureSection load='' size='350' side='right' caption='DXP reductoisomerase complex with NADPH, Mn+2 ion (green) and anti-malaria drug fosmidomycin (PDB code [[3zhy]])' scene='70/708083/Cv/1'>
== Function ==
== Function ==
'''DXP reductoisomerase''' (DXR) or '''1-deoxy-D-xylulose-5-phosphate reductoisomerase''' or '''IspC''' catalyzes the conversion of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate. DXR is part of the nonmevalonate pathway which synthesizes isoprenoids which are essential components of bacterial cell wall. NADPH is a cofactor of the reaction. Divalent metal cations (Mg<sup>+2</sup>, Mn<sup>+2</sup>) are involved in binding of the DXP substrate to DXR.<ref>PMID:9707569</ref>
'''DXP reductoisomerase''' (DXR) or '''1-deoxy-D-xylulose-5-phosphate reductoisomerase''' or '''IspC''' catalyzes the conversion of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate. DXR is part of the nonmevalonate pathway which synthesizes isoprenoids which are essential components of bacterial cell wall. NADPH is a cofactor of the reaction. Divalent metal cations (Mg<sup>+2</sup>, Mn<sup>+2</sup>) are involved in binding of the DXP substrate to DXR.<ref>PMID:9707569</ref>
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== Structural highlights ==
== Structural highlights ==
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DXR <scene name='70/708083/Cv/4'>active site</scene> contains Mn<sup>+2</sup> cation and the product analog fosmidomycin. <scene name='70/708083/Cv/7'>Click here to see NADPH binding site</scene> (water molecules shown as red spheres).<ref>PMID:23819803</ref>
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DXR <scene name='70/708083/Cv/8'>active site</scene> contains Mn<sup>+2</sup> cation and the product analog fosmidomycin. <scene name='70/708083/Cv/9'>Click here to see NADPH binding site</scene> (water molecules shown as red spheres).<ref>PMID:23819803</ref>
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</StructureSection>
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== 3D Structures of DXP reductoisomerase==
== 3D Structures of DXP reductoisomerase==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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[[DXP reductoisomerase 3D Structures]]
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*DXP reductoisomerase
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**[[1k5h]], [[1onn]] – EcDXR – ''Escherichia coli''<br />
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**[[1r0k]] – ZmDXR – ''Zymomonas mobilis''<br />
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**[[2c82]] – MtDXR – ''Mycobacterium tuberculosis''<br />
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**[[2jcy]] – MtDXR (mutant) <br />
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**[[4zn6]] – DXR – ''Acinetobacter baumannii'' <br />
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*DXP reductoisomerase binary complex
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**[[1t1r]], [[1t1s]] – EcDXR + bisphosphonate<br />
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**[[1jvs]] – EcDXR + NADPH<br />
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**DXP[[1r0l]] – ZmDXR + NADPH<br />
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**[[2jd1]] – MtDXR + NADPH<br />
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**[[2jd0]] – MtDXR (mutant) + NADPH<br />
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**[[2jd2]], [[2y1c]], [[2y1e]] – MtDXR + Mn<br />
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**[[3zhz]] – MtDXR + anti-malaria drug<br />
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**[[1ono]] – EcDXR + Mn<br />
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**[[3iie]] – DXR + Mg – ''Yersinia pestis''<br />
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*DXP reductoisomerase ternary complex
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**[[2jcv]] – MtDXR + NADPH + anti-malaria drug<br />
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**[[2y1d]], [[2y1g]], [[3zhx]], [[3zi0]] – MtDXR + Mn + anti-malaria drug<br />
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**[[2jcx]] – MtDXR (mutant) + NADPH + anti-malaria drug<br />
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**[[1onp]], [[3r0i]] – EcDXR + Mn + anti-malaria drug<br />
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**[[1q0h]], [[1q0l]] – EcDXR + NADPH + anti-malaria drug<br />
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**[[1q0q]] – EcDXR + NADPH + DXP<br />
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**[[3anl]], [[3anm]], [[3ann]] – EcDXR + NADPH + lipophilic phosphonate<br />
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**[[3a14]] – TmDXR + NADPH + Mg – ''Thermotoga maritima''<br />
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**[[3au8]] – PfDXR + NADPH + Mn – ''Plasmodium falciparum''<br />
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**[[4y67]], [[4y6p]], [[4y6r]], [[4y6s]] – PfDXR + Mn + anti-malaria drug<br />
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*DXP reductoisomerase quaternary complex
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**[[2egh]] – EcDXR + NADPH + Mg + anti-malaria drug<br />
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**[[3a06]] – TmDXR + NADPH + Mg + anti-malaria drug <br />
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**[[3au9]], [[3aua]] – PfDXR + NADPH + Mg + anti-malaria drug <br />
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**[[4kp7]] – PfDXR + NADPH + Mn + anti-malaria drug <br />
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**[[2y1f]], [[4a03]], [[4aic]], [[3zhy]] – MtDXR + NADPH + Mn + anti-malaria drug <br />
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**[[4ooe]], [[4oof]] – MtDXR (mutant) + NADPH + Mn + anti-malaria drug <br />
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**[[3ras]] – MtDXR + NADPH + Mn + lipophilic phosphonate<br />
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**[[4rcv]] – MtDXR + NADPH + Mn + 1-deoxy-L-erythrulose<br />
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**[[4gae]] – PfDXR (mutant) + NADPH + Mn + pyridine inhibitor <br />
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**[[3wqq]], [[3wqr]], [[3wqs]] – PfDXR + NADPH + Mg + inhibitor <br />
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</StructureSection>
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}}
 
== References ==
== References ==
<references/>
<references/>
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[[Category:Topic Page]]

Current revision

DXP reductoisomerase complex with NADPH, Mn+2 ion (green) and anti-malaria drug fosmidomycin (PDB code 3zhy)

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References

  1. ↑ Takahashi S, Kuzuyama T, Watanabe H, Seto H. A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis. Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9879-84. PMID:9707569
  2. ↑ Jansson AM, Wieckowska A, Bjorkelid C, Yahiaoui S, Sooriyaarachchi S, Lindh M, Bergfors T, Dharavath S, Desroses M, Suresh S, Andaloussi M, Nikhil R, Sreevalli S, Srinivasa BR, Larhed M, Jones TA, Karlen A, Mowbray SL. DXR inhibition by potent mono- and disubstituted fosmidomycin analogues. J Med Chem. 2013 Aug 8;56(15):6190-9. doi: 10.1021/jm4006498. Epub 2013 Jul 17. PMID:23819803 doi:http://dx.doi.org/10.1021/jm4006498

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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