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1rf4

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[[Image:1rf4.jpg|left|200px]]
 
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{{Structure
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==Structural Studies of Streptococcus pneumoniae EPSP Synthase, Tetrahedral intermediate Bound State==
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|PDB= 1rf4 |SIZE=350|CAPTION= <scene name='initialview01'>1rf4</scene>, resolution 2.2&Aring;
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<StructureSection load='1rf4' size='340' side='right'caption='[[1rf4]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SPQ:(3R,4S,5R)-5-{[(1R)-1-CARBOXY-2-FLUORO-1-(PHOSPHONOOXY)ETHYL]OXY}-4-HYDROXY-3-(PHOSPHONOOXY)CYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SPQ</scene>
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<table><tr><td colspan='2'>[[1rf4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RF4 FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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|GENE= aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 Streptococcus pneumoniae])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SPQ:(3R,4S,5R)-5-{[(1R)-1-CARBOXY-2-FLUORO-1-(PHOSPHONOOXY)ETHYL]OXY}-4-HYDROXY-3-(PHOSPHONOOXY)CYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SPQ</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rf4 OCA], [https://pdbe.org/1rf4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rf4 RCSB], [https://www.ebi.ac.uk/pdbsum/1rf4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rf4 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1rf5|1RF5]], [[1rf6|1RF6]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rf4 OCA], [http://www.ebi.ac.uk/pdbsum/1rf4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rf4 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/AROA_STRPN AROA_STRPN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rf/1rf4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rf4 ConSurf].
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<div style="clear:both"></div>
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'''Structural Studies of Streptococcus pneumoniae EPSP Synthase, Tetrahedral intermediate Bound State'''
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==See Also==
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*[[EPSP synthase 3D structures|EPSP synthase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The shikimate pathway synthesizes aromatic amino acids and other essential metabolites that are necessary for bacteria, plants and fungi to survive. This pathway is not present in vertebrates and therefore represents an attractive target for antibacterial agents. We have successfully crystallized and solved the structure of unliganded, inhibitor-liganded and tetrahedral intermediate (TI)-liganded forms of Streptococcus pneumoniae EPSP synthase. The overall topology of the S. pneumoniae EPSP synthase is similar to that of the Escherichia coli EPSP synthase. In addition, the majority of residues responsible for ligand binding were conserved between the two proteins. TI-liganded structure provides absolute configuration of the C-2 atom from the F-PEP moiety of the enzyme-bound intermediate and also defines key residues responsible for the enzyme reaction. Comparison of the unliganded state and substrate-bound state of the enzyme provides insights into the structural mechanisms involved in dynamic events of ligand binding, domain movement and closure. This structural study of the pathogenic bacteria S. pneumoniae EPSP synthase with inhibitor and TI will provide invaluable information for the design of new-generation antibiotics.
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[[Category: Large Structures]]
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==About this Structure==
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1RF4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RF4 OCA].
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==Reference==
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Structural studies of Streptococcus pneumoniae EPSP synthase in unliganded state, tetrahedral intermediate-bound state and S3P-GLP-bound state., Park H, Hilsenbeck JL, Kim HJ, Shuttleworth WA, Park YH, Evans JN, Kang C, Mol Microbiol. 2004 Feb;51(4):963-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14763973 14763973]
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[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
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[[Category: Single protein]]
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[[Category: Streptococcus pneumoniae]]
[[Category: Streptococcus pneumoniae]]
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[[Category: Evans, J N.]]
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[[Category: Evans JN]]
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[[Category: Hilsenbeck, J L.]]
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[[Category: Hilsenbeck JL]]
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[[Category: Kang, C.]]
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[[Category: Kang C]]
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[[Category: Kim, H J.]]
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[[Category: Kim HJ]]
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[[Category: Park, H.]]
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[[Category: Park H]]
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[[Category: Park, Y H.]]
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[[Category: Park YH]]
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[[Category: Shuttleworth, W A.]]
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[[Category: Shuttleworth WA]]
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[[Category: epsp synthase]]
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[[Category: glyphosate]]
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[[Category: pep]]
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[[Category: s. pneumoniae]]
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[[Category: s3p]]
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[[Category: shikimate pathway]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:27:17 2008''
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Current revision

Structural Studies of Streptococcus pneumoniae EPSP Synthase, Tetrahedral intermediate Bound State

PDB ID 1rf4

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