4q2j

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==A novel structure-based mechanism for DNA-binding of SATB1==
==A novel structure-based mechanism for DNA-binding of SATB1==
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<StructureSection load='4q2j' size='340' side='right' caption='[[4q2j]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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<StructureSection load='4q2j' size='340' side='right'caption='[[4q2j]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4q2j]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q2J FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4q2j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q2J FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2j OCA], [http://pdbe.org/4q2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q2j RCSB], [http://www.ebi.ac.uk/pdbsum/4q2j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q2j ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.603&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2j OCA], [https://pdbe.org/4q2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q2j RCSB], [https://www.ebi.ac.uk/pdbsum/4q2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q2j ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/SATB1_MOUSE SATB1_MOUSE]] Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis (By similarity). Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes.<ref>PMID:10716941</ref> <ref>PMID:11463840</ref> <ref>PMID:12692553</ref> <ref>PMID:15814699</ref> <ref>PMID:17057718</ref> <ref>PMID:18722016</ref> <ref>PMID:19103759</ref> <ref>PMID:19386260</ref> <ref>PMID:9271405</ref>
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[https://www.uniprot.org/uniprot/SATB1_MOUSE SATB1_MOUSE] Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis (By similarity). Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes.<ref>PMID:10716941</ref> <ref>PMID:11463840</ref> <ref>PMID:12692553</ref> <ref>PMID:15814699</ref> <ref>PMID:17057718</ref> <ref>PMID:18722016</ref> <ref>PMID:19103759</ref> <ref>PMID:19386260</ref> <ref>PMID:9271405</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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SATB1 is essential for T-cell development and growth and metastasis of multitype tumors and acts as a global chromatin organizer and gene expression regulator. The DNA binding ability of SATB1 plays vital roles in its various biological functions. We report the crystal structure of the N-terminal module of SATB1. Interestingly, this module contains a ubiquitin-like domain (ULD) and a CUT repeat-like (CUTL) domain (ULD-CUTL tandem). Detailed biochemical experiments indicate that the N terminus of SATB1 (residues 1-248, SATB1((1-248))), including the extreme 70 N-terminal amino acids, and the ULD-CUTL tandem bind specifically to DNA targets. Our results show that the DNA binding ability of full-length SATB1 requires the contribution of the CUTL domain, as well as the CUT1-CUT2 tandem domain and the homeodomain. These findings may reveal a multiple-domain-coordinated mechanism whereby SATB1 recognizes DNA targets.
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Crystal structure of the ubiquitin-like domain-CUT repeat-like tandem of special AT-rich sequence binding protein 1 (SATB1) reveals a coordinating DNA-binding mechanism.,Wang Z, Yang X, Guo S, Yang Y, Su XC, Shen Y, Long J J Biol Chem. 2014 Oct 3;289(40):27376-85. doi: 10.1074/jbc.M114.562314. Epub 2014, Aug 14. PMID:25124042<ref>PMID:25124042</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4q2j" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Long, J]]
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[[Category: Large Structures]]
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[[Category: Shen, Y]]
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[[Category: Mus musculus]]
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[[Category: Wang, Z]]
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[[Category: Long J]]
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[[Category: Yang, X]]
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[[Category: Shen Y]]
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[[Category: Dna binding protein]]
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[[Category: Wang Z]]
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[[Category: Yang X]]

Current revision

A novel structure-based mechanism for DNA-binding of SATB1

PDB ID 4q2j

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