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1ri1

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[[Image:1ri1.jpg|left|200px]]
 
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{{Structure
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==Structure and mechanism of mRNA cap (guanine N-7) methyltransferase==
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|PDB= 1ri1 |SIZE=350|CAPTION= <scene name='initialview01'>1ri1</scene>, resolution 2.50&Aring;
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<StructureSection load='1ri1' size='340' side='right'caption='[[1ri1]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5&#39;-TRIPHOSPHATE-5&#39;-GUANOSINE'>GTG</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
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<table><tr><td colspan='2'>[[1ri1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. The January 2012 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Messenger RNA Capping'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2012_1 10.2210/rcsb_pdb/mom_2012_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RI1 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE= ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 Encephalitozoon cuniculi])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ri1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri1 OCA], [https://pdbe.org/1ri1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ri1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ri1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ri1 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1ri2|1RI2]], [[1ri3|1RI3]], [[1ri4|1RI4]], [[1ri5|1RI5]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ri1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri1 OCA], [http://www.ebi.ac.uk/pdbsum/1ri1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ri1 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/MCES_ENCCU MCES_ENCCU] mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.<ref>PMID:15760890</ref>
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== Evolutionary Conservation ==
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'''Structure and mechanism of mRNA cap (guanine N-7) methyltransferase'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ri/1ri1_consurf.spt"</scriptWhenChecked>
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A suite of crystal structures is reported for a cellular mRNA cap (guanine-N7) methyltransferase in complex with AdoMet, AdoHcy, and the cap guanylate. Superposition of ligand complexes suggests an in-line mechanism of methyl transfer, albeit without direct contacts between the enzyme and either the N7 atom of guanine (the attacking nucleophile), the methyl carbon of AdoMet, or the sulfur of AdoMet/AdoHcy (the leaving group). The structures indicate that catalysis of cap N7 methylation is accomplished by optimizing proximity and orientation of the substrates, assisted by a favorable electrostatic environment. The enzyme-ligand structures, together with new mutational data, fully account for the biochemical specificity of the cap guanine-N7 methylation reaction, an essential and defining step of eukaryotic mRNA synthesis.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1RI1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI1 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ri1 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Structure and mechanism of mRNA cap (guanine-N7) methyltransferase., Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD, Mol Cell. 2004 Jan 16;13(1):77-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14731396 14731396]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Encephalitozoon cuniculi]]
[[Category: Encephalitozoon cuniculi]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fabrega, C.]]
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[[Category: Messenger RNA Capping]]
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[[Category: Hausmann, S.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Lima, C D.]]
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[[Category: Fabrega C]]
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[[Category: Shen, V.]]
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[[Category: Hausmann S]]
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[[Category: Shuman, S.]]
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[[Category: Lima CD]]
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[[Category: cap]]
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[[Category: Shen V]]
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[[Category: m7g]]
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[[Category: Shuman S]]
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[[Category: messenger rna cap]]
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[[Category: methyltransferase]]
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[[Category: rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:28:27 2008''
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Current revision

Structure and mechanism of mRNA cap (guanine N-7) methyltransferase

PDB ID 1ri1

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