1rhy

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[[Image:1rhy.gif|left|200px]]
 
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{{Structure
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==Crystal structure of Imidazole Glycerol Phosphate Dehydratase==
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|PDB= 1rhy |SIZE=350|CAPTION= <scene name='initialview01'>1rhy</scene>, resolution 2.30&Aring;
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<StructureSection load='1rhy' size='340' side='right'caption='[[1rhy]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=EMC:ETHYL+MERCURY+ION'>EMC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1rhy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryptococcus_neoformans Cryptococcus neoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RHY FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Imidazoleglycerol-phosphate_dehydratase Imidazoleglycerol-phosphate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.19 4.2.1.19] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE= HIS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5207 Filobasidiella neoformans])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=EMC:ETHYL+MERCURY+ION'>EMC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rhy OCA], [https://pdbe.org/1rhy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rhy RCSB], [https://www.ebi.ac.uk/pdbsum/1rhy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rhy ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rhy OCA], [http://www.ebi.ac.uk/pdbsum/1rhy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rhy RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/HIS7_CRYNJ HIS7_CRYNJ] Imidazole glycerol-phosphate dehydratase required for histidine biosynthesis.<ref>PMID:8045413</ref>
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== Evolutionary Conservation ==
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'''Crystal structure of Imidazole Glycerol Phosphate Dehydratase'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rh/1rhy_consurf.spt"</scriptWhenChecked>
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Imidazole glycerol-phosphate dehydratase (IGPD) catalyzes the sixth step of histidine biosynthesis. The enzyme is of fundamental biochemical interest, because it catalyzes removal of a non-acidic hydrogen atom in the dehydration reaction. It is also a potential target for development of herbicides. IGPD is a metalloenzyme in which transition metals induce aggregation and are required for catalysis. Addition of 1 equivalent of Mn(2+)/subunit is shown by analytical ultracentrifugation to induce the formation of 24-mers from trimeric IGPD. Two histidine-rich motifs may participate in metal binding and aggregation. The 2.3-A crystal structure of metal-free trimeric IGPD from the fungus Filobasidiella neoformans reveals a novel fold containing an internal repeat, apparently the result of gene duplication. The 95-residue alpha/beta half-domain occurs in a few other proteins, including the GHMP kinase superfamily (galacto-homoserine-mevalonate-phosphomevalonate), but duplication to form a compact domain has not been seen elsewhere. Conserved residues cluster at two types of sites in the trimer, each site containing a conserved histidine-rich motif. A model is proposed for the intact, active 24-mer in which all highly conserved residues, including the histidine-rich motifs in both the N- and C-terminal halves of the polypeptide, cluster at a common site between trimers. This site is a candidate for the active site and also for metal binding leading to aggregation of trimers. The structure provides a basis for further studies of enzyme function and mechanism and for development of more potent and specific herbicides.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1RHY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Filobasidiella_neoformans Filobasidiella neoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHY OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rhy ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Crystal structure of imidazole glycerol-phosphate dehydratase: duplication of an unusual fold., Sinha SC, Chaudhuri BN, Burgner JW, Yakovleva G, Davisson VJ, Smith JL, J Biol Chem. 2004 Apr 9;279(15):15491-8. Epub 2004 Jan 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14724278 14724278]
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<references/>
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[[Category: Filobasidiella neoformans]]
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__TOC__
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[[Category: Imidazoleglycerol-phosphate dehydratase]]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Cryptococcus neoformans]]
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[[Category: Burgner, J W.]]
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[[Category: Large Structures]]
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[[Category: Chaudhuri, B N.]]
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[[Category: Burgner JW]]
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[[Category: Davisson, V J.]]
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[[Category: Chaudhuri BN]]
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[[Category: Sinha, S C.]]
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[[Category: Davisson VJ]]
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[[Category: Smith, J L.]]
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[[Category: Sinha SC]]
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[[Category: Yakovleva, G.]]
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[[Category: Smith JL]]
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[[Category: dehydratase]]
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[[Category: Yakovleva G]]
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[[Category: gene duplication]]
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[[Category: histidine biosynthesis]]
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[[Category: left-handed b-a-b crossover motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:28:30 2008''
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Current revision

Crystal structure of Imidazole Glycerol Phosphate Dehydratase

PDB ID 1rhy

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