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5ugq
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5ugq is ON HOLD Authors: Naffin-Olivos, J.L., Daab, A., White, A., Goldfarb, N., Milne, A.C., Liu, D., Dunn, B.M., Rengarajan, J., Petsko, G.A., Rin...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of Hip1 (Rv2224c)== | |
| + | <StructureSection load='5ugq' size='340' side='right'caption='[[5ugq]], [[Resolution|resolution]] 2.61Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5ugq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UGQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UGQ FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">caeA, Rv2224c, MTCY427.05c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ugq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ugq OCA], [http://pdbe.org/5ugq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ugq RCSB], [http://www.ebi.ac.uk/pdbsum/5ugq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ugq ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/CAEA_MYCTU CAEA_MYCTU]] Involved in virulence. Catalyzes the cleavage of ester bonds. Esterase activity increases with increasing carbon chain length of the substrate.<ref>PMID:17428787</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Mycobacterium tuberculosis (Mtb) serine protease Hip1 (hydrolase important for pathogenesis; Rv2224c) promotes tuberculosis (TB) pathogenesis by impairing host immune responses through proteolysis of a protein substrate, Mtb GroEL2. The cell surface localization of Hip1 and its immunomodulatory functions make Hip1 a good drug target for new adjunctive immune therapies for TB. Here, we report the crystal structure of Hip1 to a resolution of 2.6 A and the kinetic studies of the enzyme against model substrates and the protein GroEL2. The structure shows a two-domain protein, one of which contains the catalytic residues that are the signature of a serine protease. Surprisingly, a threonine is located within the active site close enough to hydrogen bond with the catalytic residues Asp463 and His490. Mutation of this residue, Thr466, to alanine established its importance for function. Our studies provide insights into the structure of a member of a novel family of proteases. Knowledge of the Hip1 structure will aid in designing inhibitors that could block Hip1 activity. | ||
| - | + | Structure Determination of Mycobacterium tuberculosis Serine Protease Hip1 (Rv2224c).,Naffin-Olivos JL, Daab A, White A, Goldfarb NE, Milne AC, Liu D, Baikovitz J, Dunn BM, Rengarajan J, Petsko GA, Ringe D Biochemistry. 2017 Apr 7. doi: 10.1021/acs.biochem.6b01066. PMID:28346784<ref>PMID:28346784</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[ | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5ugq" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: Dunn, B | + | *[[Carboxylesterase 3D structures|Carboxylesterase 3D structures]] |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Myctu]] | ||
| + | [[Category: Daab, A]] | ||
| + | [[Category: Dunn, B M]] | ||
| + | [[Category: Goldfarb, N]] | ||
[[Category: Liu, D]] | [[Category: Liu, D]] | ||
| + | [[Category: Milne, A C]] | ||
| + | [[Category: Naffin-Olivos, J L]] | ||
| + | [[Category: Petsko, G A]] | ||
[[Category: Rengarajan, J]] | [[Category: Rengarajan, J]] | ||
[[Category: Ringe, D]] | [[Category: Ringe, D]] | ||
| - | [[Category: | + | [[Category: White, A]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| + | [[Category: Serine protease]] | ||
Current revision
Crystal Structure of Hip1 (Rv2224c)
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Categories: Large Structures | Myctu | Daab, A | Dunn, B M | Goldfarb, N | Liu, D | Milne, A C | Naffin-Olivos, J L | Petsko, G A | Rengarajan, J | Ringe, D | White, A | Hydrolase | Serine protease
