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| | ==Crystal structure of Chlamydia trachomatis DsbA== | | ==Crystal structure of Chlamydia trachomatis DsbA== |
| - | <StructureSection load='5kbc' size='340' side='right' caption='[[5kbc]], [[Resolution|resolution]] 2.71Å' scene=''> | + | <StructureSection load='5kbc' size='340' side='right'caption='[[5kbc]], [[Resolution|resolution]] 2.71Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5kbc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KBC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KBC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5kbc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_trachomatis_A2497 Chlamydia trachomatis A2497]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KBC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KBC FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kbc OCA], [http://pdbe.org/5kbc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kbc RCSB], [http://www.ebi.ac.uk/pdbsum/5kbc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kbc ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.706Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kbc OCA], [https://pdbe.org/5kbc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kbc RCSB], [https://www.ebi.ac.uk/pdbsum/5kbc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kbc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/G4NNC6_CHLT4 G4NNC6_CHLT4] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Groftehauge, M K]] | + | [[Category: Chlamydia trachomatis A2497]] |
| - | [[Category: Martin, J L]] | + | [[Category: Large Structures]] |
| - | [[Category: McMahon, R M]] | + | [[Category: Groftehauge MK]] |
| - | [[Category: Oxidoreductase]] | + | [[Category: Martin JL]] |
| - | [[Category: Thioredoxin fold]] | + | [[Category: McMahon RM]] |
| Structural highlights
Function
G4NNC6_CHLT4
Publication Abstract from PubMed
The Gram negative bacteria Chlamydia trachomatis is an obligate intracellular human pathogen that can cause pelvic inflammatory disease, infertility and blinding trachoma. C. trachomatis encodes a homolog of the dithiol oxidoreductase DsbA. Bacterial DsbA proteins introduce disulfide bonds to folding proteins providing structural bracing for secreted virulence factors, consequently these proteins are potential targets for antimicrobial drugs. Despite sharing functional and structural characteristics, the DsbA enzymes studied to date vary widely in their redox character. In this study we show that the truncated soluble form of the predicted membrane anchored protein C. trachomatis DsbA (CtDsbA) has oxidase activity and redox properties broadly similar to other characterized DsbA proteins. However CtDsbA is distinguished from other DsbAs by having six cysteines, including a second disulfide bond, and an unusual dipeptide sequence in its catalytic motif (Cys-Ser-Ala-Cys). We report the 2.7 A crystal structure of CtDsbA revealing a typical DsbA fold, which is most similar to that of DsbA-II type proteins. Consistent with this, the catalytic surface of CtDsbA is negatively charged and lacks the hydrophobic groove found in EcDsbA and DsbAs from other enterobacteriaceae. Biochemical characterization of CtDsbA reveals it to be weakly oxidizing compared to other DsbAs and with only a mildly destabilizing active site disulfide bond. Analysis of the crystal structure suggests that this redox character is consistent with a lack of contributing factors to stabilize the active site nucleophilic thiolate relative to more oxidizing DsbA proteins.
Structural and Biochemical Characterization of Chlamydia trachomatis DsbA Reveals a Cysteine-Rich and Weakly Oxidising Oxidoreductase.,Christensen S, Groftehauge MK, Byriel K, Huston WM, Furlong E, Heras B, Martin JL, McMahon RM PLoS One. 2016 Dec 28;11(12):e0168485. doi: 10.1371/journal.pone.0168485., eCollection 2016. PMID:28030602[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Christensen S, Groftehauge MK, Byriel K, Huston WM, Furlong E, Heras B, Martin JL, McMahon RM. Structural and Biochemical Characterization of Chlamydia trachomatis DsbA Reveals a Cysteine-Rich and Weakly Oxidising Oxidoreductase. PLoS One. 2016 Dec 28;11(12):e0168485. doi: 10.1371/journal.pone.0168485., eCollection 2016. PMID:28030602 doi:http://dx.doi.org/10.1371/journal.pone.0168485
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