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4rhe

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==Crystal structure of UbiX, an aromatic acid decarboxylase from the Colwellia psychrerythraea 34H==
==Crystal structure of UbiX, an aromatic acid decarboxylase from the Colwellia psychrerythraea 34H==
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<StructureSection load='4rhe' size='340' side='right' caption='[[4rhe]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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<StructureSection load='4rhe' size='340' side='right'caption='[[4rhe]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4rhe]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RHE FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4rhe]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Colwellia_psychrerythraea_34H Colwellia psychrerythraea 34H]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RHE FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.003&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4rhf|4rhf]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rhe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhe OCA], [http://pdbe.org/4rhe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rhe RCSB], [http://www.ebi.ac.uk/pdbsum/4rhe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rhe ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rhe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhe OCA], [https://pdbe.org/4rhe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rhe RCSB], [https://www.ebi.ac.uk/pdbsum/4rhe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rhe ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q489U8_COLP3 Q489U8_COLP3]
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The ubiX gene of Colwellia psychrerythraea strain 34H encodes a 3-octaprenyl-4-hydroxybenzoate carboxylase (CpsUbiX, UniProtKB code: Q489U8) that is involved in the third step of the ubiquinone biosynthesis pathway and harbors a flavin mononucleotide (FMN) as a potential cofactor. Here, we report the crystal structures of two forms of CpsUbiX: an FMN-bound wild type form and an FMN-unbound V47S mutant form. CpsUbiX is a dodecameric enzyme, and each monomer possesses a typical Rossmann-fold structure. The FMN-binding domain of UbiX is composed of three neighboring subunits. The highly conserved Gly15, Ser41, Val47, and Tyr171 residues play important roles in FMN binding. Structural comparison of the FMN-bound wild type form with the FMN-free form reveals a significant conformational difference in the C-terminal loop region (comprising residues 170-176 and 195-206). Subsequent computational modeling and liposome binding assay both suggest that the conformational flexibility observed in the C-terminal loops plays an important role in substrate and lipid bindings. The crystal structures presented in this work provide structural framework and insights into the catalytic mechanism of CpsUbiX.
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Crystal structure of UbiX, an aromatic acid decarboxylase from the psychrophilic bacterium Colwellia psychrerythraea that undergoes FMN-induced conformational changes.,Do H, Kim SJ, Lee CW, Kim HW, Park HH, Kim HM, Park H, Park H, Lee JH Sci Rep. 2015 Feb 3;5:8196. doi: 10.1038/srep08196. PMID:25645665<ref>PMID:25645665</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4rhe" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Do, H]]
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[[Category: Colwellia psychrerythraea 34H]]
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[[Category: Kim, H M]]
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[[Category: Large Structures]]
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[[Category: Kim, H W]]
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[[Category: Do H]]
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[[Category: Kim, S J]]
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[[Category: Kim H-W]]
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[[Category: Lee, C W]]
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[[Category: Kim HM]]
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[[Category: Lee, J H]]
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[[Category: Kim SJ]]
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[[Category: Park, H]]
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[[Category: Lee CW]]
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[[Category: Park, H H]]
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[[Category: Lee JH]]
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[[Category: Park, H J]]
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[[Category: Park H]]
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[[Category: Decarboxylase]]
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[[Category: Park HH]]
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[[Category: Lyase]]
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[[Category: Park HJ]]
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[[Category: Rossmann fold]]
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Current revision

Crystal structure of UbiX, an aromatic acid decarboxylase from the Colwellia psychrerythraea 34H

PDB ID 4rhe

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