4rih

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==Chimeric Glycosyltransferase LanGT2S8Ac, carbasugar substrate complex==
==Chimeric Glycosyltransferase LanGT2S8Ac, carbasugar substrate complex==
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<StructureSection load='4rih' size='340' side='right' caption='[[4rih]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
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<StructureSection load='4rih' size='340' side='right'caption='[[4rih]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4rih]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RIH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RIH FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4rih]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_cyanogenus Streptomyces cyanogenus] and [https://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RIH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RIH FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3R2:2-DEOXY-5-O-[(R)-{[(R)-{[(1S,3R,4R,5S)-3,4-DIHYDROXY-5-METHYLCYCLOHEXYL]OXY}(HYDROXY)PHOSPHORYL]OXY}(HYDROXY)PHOSPHORYL]-3,4-DIHYDROTHYMIDINE'>3R2</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.22&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rih OCA], [http://pdbe.org/4rih PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rih RCSB], [http://www.ebi.ac.uk/pdbsum/4rih PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rih ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3R2:2-DEOXY-5-O-[(R)-{[(R)-{[(1S,3R,4R,5S)-3,4-DIHYDROXY-5-METHYLCYCLOHEXYL]OXY}(HYDROXY)PHOSPHORYL]OXY}(HYDROXY)PHOSPHORYL]-3,4-DIHYDROTHYMIDINE'>3R2</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rih OCA], [https://pdbe.org/4rih PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rih RCSB], [https://www.ebi.ac.uk/pdbsum/4rih PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rih ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q9ZGC0_STRCY Q9ZGC0_STRCY] [https://www.uniprot.org/uniprot/Q9RPA7_STRFR Q9RPA7_STRFR]
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The structures of the O-glycosyltransferase LanGT2 and the engineered, CC bond-forming variant LanGT2S8Ac show how the replacement of a single loop can change the functionality of the enzyme. Crystal structures of the enzymes in complex with a nonhydrolyzable nucleotide-sugar analogue revealed that there is a conformational transition to create the binding sites for the aglycon substrate. This induced-fit transition was explored by molecular docking experiments with various aglycon substrates.
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Structural Characterization of O- and C-Glycosylating Variants of the Landomycin Glycosyltransferase LanGT2.,Tam HK, Harle J, Gerhardt S, Rohr J, Wang G, Thorson JS, Bigot A, Lutterbeck M, Seiche W, Breit B, Bechthold A, Einsle O Angew Chem Int Ed Engl. 2015 Jan 7. doi: 10.1002/anie.201409792. PMID:25581707<ref>PMID:25581707</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4rih" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bechthold, A]]
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[[Category: Large Structures]]
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[[Category: Breit, B]]
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[[Category: Streptomyces cyanogenus]]
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[[Category: Einsle, O]]
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[[Category: Streptomyces fradiae]]
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[[Category: Gerhardt, S]]
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[[Category: Bechthold A]]
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[[Category: Tam, H K]]
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[[Category: Breit B]]
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[[Category: C-glycosylating glycosyltransferase]]
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[[Category: Einsle O]]
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[[Category: Gt fold]]
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[[Category: Gerhardt S]]
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[[Category: Transferase]]
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[[Category: Tam HK]]

Current revision

Chimeric Glycosyltransferase LanGT2S8Ac, carbasugar substrate complex

PDB ID 4rih

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