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1rsn

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[[Image:1rsn.gif|left|200px]]
 
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{{Structure
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==RIBONUCLEASE (RNASE SA) (E.C.3.1.4.8) COMPLEXED WITH EXO-2',3'-CYCLOPHOSPHOROTHIOATE==
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|PDB= 1rsn |SIZE=350|CAPTION= <scene name='initialview01'>1rsn</scene>, resolution 2.0&Aring;
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<StructureSection load='1rsn' size='340' side='right'caption='[[1rsn]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SGP:GUANOSINE-2&#39;,3&#39;-CYCLOPHOSPHOROTHIOATE'>SGP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1rsn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kitasatospora_aureofaciens Kitasatospora aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RSN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RSN FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SGP:GUANOSINE-2,3-CYCLOPHOSPHOROTHIOATE'>SGP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rsn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rsn OCA], [https://pdbe.org/1rsn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rsn RCSB], [https://www.ebi.ac.uk/pdbsum/1rsn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rsn ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rsn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rsn OCA], [http://www.ebi.ac.uk/pdbsum/1rsn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rsn RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/RNSA_KITAU RNSA_KITAU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rs/1rsn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rsn ConSurf].
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<div style="clear:both"></div>
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'''RIBONUCLEASE (RNASE SA) (E.C.3.1.4.8) COMPLEXED WITH EXO-2',3'-CYCLOPHOSPHOROTHIOATE'''
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==See Also==
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The structure of the complex of ribonuclease from Streptomyces aureofaciens (RNase Sa) with exo guanosine 2',3'-cyclophosphorothioate has been refined against 0.2-nm resolution synchrotron data using, as a starting model, coordinates from the RNase Sa: 2'-GMP complex. The refinement was based on all data over 1.0-0.2 nm and converged to a crystallographic R factor of 11.9%. This is the first structure of a microbial ribonuclease complexed with a 2',3'-cyclophosphorothioate, which is a thio analogue of the intermediate of the two-step reaction. However, exo guanosine 2',3'-cyclophosphorothioate is bound in a non-functional mode and is not hydrolysed. This structure therefore does not provide direct evidence on the identity of the amino acid residues responsible for catalytic cleavage of the substrate. However, based on present and previous results, a plausible model is proposed for the complex of the cyclic intermediate which acts as substrate for the second step of the catalysis.
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[[Category: Kitasatospora aureofaciens]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Dauter Z]]
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1RSN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RSN OCA].
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[[Category: Lamzin VS]]
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[[Category: Sevcik J]]
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==Reference==
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[[Category: Wilson KS]]
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Complex of ribonuclease Sa with a cyclic nucleotide and a proposed model for the reaction intermediate., Sevcik J, Zegers I, Wyns L, Dauter Z, Wilson KS, Eur J Biochem. 1993 Aug 15;216(1):301-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8396032 8396032]
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[[Category: Ribonuclease T(1)]]
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[[Category: Single protein]]
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[[Category: Streptomyces aureofaciens]]
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[[Category: Dauter, Z.]]
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[[Category: Lamzin, V S.]]
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[[Category: Sevcik, J.]]
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[[Category: Wilson, K S.]]
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[[Category: hydrolase (guanyloribonuclease)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:32:22 2008''
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Current revision

RIBONUCLEASE (RNASE SA) (E.C.3.1.4.8) COMPLEXED WITH EXO-2',3'-CYCLOPHOSPHOROTHIOATE

PDB ID 1rsn

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