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5fyr

From Proteopedia

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Calcium-dependent phosphoinositol-specific phospholipase C from a Gram-negative bacterium, Pseudomonas sp, apo form, myoinositol complex

Structural highlights

5fyr is a 4 chain structure with sequence from Pseudomonas sp.. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.45Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A1S4NYD4_PSESP

Publication Abstract from PubMed

Bacterial phosphoinositide-specific phospholipases C (PI-PLCs) are the smallest members of the PI-PLC family, which includes much larger mammalian enzymes responsible for signal transduction as well as enzymes from protozoan parasites, yeast and plants. Eukaryotic PI-PLCs have calcium in the active site, but this is absent in the known structures of Gram-positive bacteria, where its role is instead played by arginine. In addition to their use in a number of industrial applications, the bacterial enzymes attract special interest because they can serve as convenient models of the catalytic domains of eukaryotic enzymes for in vitro activity studies. Here, the structure of a PI-PLC from Pseudomonas sp. 62186 is reported, the first from a Gram-negative bacterium and the first of a native bacterial PI-PLC with calcium present in the active site. Solution of the structure posed particular problems owing to the low sequence identity of available homologous structures. Its dependence on calcium for catalysis makes this enzyme a better model for studies of the mammalian PI-PLCs than the previously used calcium-independent bacterial PI-PLCs.

The structure of a calcium-dependent phosphoinositide-specific phospholipase C from Pseudomonas sp. 62186, the first from a Gram-negative bacterium.,Moroz OV, Blagova E, Lebedev AA, Norgaard A, Segura DR, Blicher TH, Brask J, Wilson KS Acta Crystallogr D Struct Biol. 2017 Jan 1;73(Pt 1):32-44. doi:, 10.1107/S2059798316019616. Epub 2017 Jan 1. PMID:28045383^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Moroz OV, Blagova E, Lebedev AA, Norgaard A, Segura DR, Blicher TH, Brask J, Wilson KS. The structure of a calcium-dependent phosphoinositide-specific phospholipase C from Pseudomonas sp. 62186, the first from a Gram-negative bacterium. Acta Crystallogr D Struct Biol. 2017 Jan 1;73(Pt 1):32-44. doi:, 10.1107/S2059798316019616. Epub 2017 Jan 1. PMID:28045383 doi:http://dx.doi.org/10.1107/S2059798316019616

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5fyr"

@@ Line 1: / Line 1: @@
 ==Calcium-dependent phosphoinositol-specific phospholipase C from a Gram-negative bacterium, Pseudomonas sp, apo form, myoinositol complex==
-<StructureSection load='5fyr' size='340' side='right' caption='[[5fyr]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
+<StructureSection load='5fyr' size='340' side='right'caption='[[5fyr]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5fyr]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FYR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FYR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5fyr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FYR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FYR FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=INS:1,2,3,4,5,6-HEXAHYDROXY-CYCLOHEXANE'>INS</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fyo|5fyo]], [[5fyp|5fyp]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=INS:1,2,3,4,5,6-HEXAHYDROXY-CYCLOHEXANE'>INS</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fyr OCA], [http://pdbe.org/5fyr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fyr RCSB], [http://www.ebi.ac.uk/pdbsum/5fyr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fyr ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fyr OCA], [https://pdbe.org/5fyr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fyr RCSB], [https://www.ebi.ac.uk/pdbsum/5fyr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fyr ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A1S4NYD4_PSESP A0A1S4NYD4_PSESP]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 19: @@
 </div>
 <div class="pdbe-citations 5fyr" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Phospholipase C|Phospholipase C]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Blagova, E]]
+[[Category: Large Structures]]
-[[Category: Blicher, T H]]
+[[Category: Pseudomonas sp]]
-[[Category: Lebedev, A A]]
+[[Category: Blagova E]]
-[[Category: Moroz, O V]]
+[[Category: Blicher TH]]
-[[Category: Norgaard, A]]
+[[Category: Lebedev AA]]
-[[Category: Segura, D R]]
+[[Category: Moroz OV]]
-[[Category: Wilson, K S]]
+[[Category: Norgaard A]]
-[[Category: Bacterial]]
+[[Category: Segura DR]]
-[[Category: Calcium-dependent]]
+[[Category: Wilson KS]]
-[[Category: Gram-negative]]
-[[Category: Hydrolase]]
-[[Category: Myoinositol complex]]
-[[Category: Pi-plc]]
-[[Category: Pseudomona]]

5fyr

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Current revision

Calcium-dependent phosphoinositol-specific phospholipase C from a Gram-negative bacterium, Pseudomonas sp, apo form, myoinositol complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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