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5jnh

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==Crystal Structure of cytidine monophosphate hydroxymethylase MilA==
==Crystal Structure of cytidine monophosphate hydroxymethylase MilA==
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<StructureSection load='5jnh' size='340' side='right' caption='[[5jnh]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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<StructureSection load='5jnh' size='340' side='right'caption='[[5jnh]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5jnh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JNH FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5jnh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_rimofaciens Streptomyces rimofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JNH FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5jp9|5jp9]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.202&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jnh OCA], [http://pdbe.org/5jnh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jnh RCSB], [http://www.ebi.ac.uk/pdbsum/5jnh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jnh ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jnh OCA], [https://pdbe.org/5jnh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jnh RCSB], [https://www.ebi.ac.uk/pdbsum/5jnh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jnh ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/B4Y380_9ACTN B4Y380_9ACTN]
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Modified pyrimidine monophosphates such as methyl dCMP (mdCMP), hydroxymethyl dUMP (hmdUMP) and hmdCMP in some phages are synthesized by a large group of enzymes termed as thymidylate synthases (TS). Thymidylate is a nucleotide required for DNA synthesis and thus TS is an important drug target. In the biosynthetic pathway of the nucleoside fungicide mildiomycin isolated from Streptomyces rimofaciens ZJU5119, a cytidylate (CMP) hydroxymethylase, MilA, catalyzes the conversion of CMP into 5'-hydroxymethyl CMP (hmCMP) with an efficiency (kcat/KM) of 5-fold faster than for deoxycytidylate (dCMP). MilA is thus the first enzyme of the TS superfamily preferring CMP to dCMP. Here, we determined the crystal structures of MilA and its complexes with various substrates including CMP, dCMP and hmCMP. Comparing these structures to those of dCMP hydroxymethylase (CH) from T4 phage and TS from Escherichia coli revealed that two residues in the active site of CH and TS, a serine and an arginine, are respectively replaced by an alanine and a lysine, Ala176 and Lys133, in MilA. Mutation of A176S/K133R of MilA resulted in a reversal of substrate preference from CMP to dCMP. This is the first study reporting the evolution of the conserved TS in substrate selection from DNA metabolism to secondary nucleoside biosynthesis.
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Structural basis of the substrate preference towards CMP for a thymidylate synthase MilA involved in mildiomycin biosynthesis.,Zhao G, Chen C, Xiong W, Gao T, Deng Z, Wu G, He X Sci Rep. 2016 Dec 21;6:39675. doi: 10.1038/srep39675. PMID:28000775<ref>PMID:28000775</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5jnh" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: He, X]]
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[[Category: Large Structures]]
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[[Category: Zhao, G]]
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[[Category: Streptomyces rimofaciens]]
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[[Category: Cmp hydroxymethylase]]
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[[Category: He X]]
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[[Category: Transferase]]
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[[Category: Zhao G]]

Current revision

Crystal Structure of cytidine monophosphate hydroxymethylase MilA

PDB ID 5jnh

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