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| - | [[Image:1s23.gif|left|200px]] | |
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| - | {{Structure
| + | ==Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG== |
| - | |PDB= 1s23 |SIZE=350|CAPTION= <scene name='initialview01'>1s23</scene>, resolution 1.60Å
| + | <StructureSection load='1s23' size='340' side='right'caption='[[1s23]], [[Resolution|resolution]] 1.60Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| + | <table><tr><td colspan='2'>[[1s23]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S23 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s23 OCA], [https://pdbe.org/1s23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s23 RCSB], [https://www.ebi.ac.uk/pdbsum/1s23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s23 ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s23 OCA], [http://www.ebi.ac.uk/pdbsum/1s23 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s23 RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG'''
| + | [[Category: Murshudov GN]] |
| - | | + | [[Category: Steiner RA]] |
| - | | + | [[Category: Subirana JA]] |
| - | ==Overview==
| + | [[Category: Valls N]] |
| - | The deoxyoligonucleotide d(CGCAATTGCG) has previously been crystallized in four different space groups. The crystals diffract to moderate resolution (2.3-2.9 A). Here, a fifth crystal form that diffracts to higher resolution (1.6 A) is presented which was obtained thanks to the use of Co2+ and cryogenic temperatures. The availability of five different crystal structures allows a thorough analysis of the conformational variability of this DNA sequence. It is concluded that the central hexamer sequence CAATTG has a practically constant conformation under all conditions, whilst the terminal base pairs at both ends vary considerably as a result of differing interactions in the crystals. The new crystal structure presented here is stabilized by guanine-Co2+-guanine interactions and the formation of C1+ -G8.C3 triplexes between neighbouring duplexes. As a result of the higher resolution of the crystal structure, a more regular structure was obtained and a clear definition of the spine of hydration was observed which was not visible in the four previous structures.
| + | [[Category: Wright G]] |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1S23 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S23 OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | DNA variability in five crystal structures of d(CGCAATTGCG)., Valls N, Wright G, Steiner RA, Murshudov GN, Subirana JA, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):680-5. Epub 2004, Mar 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15039556 15039556]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Murshudov, G N.]] | + | |
| - | [[Category: Steiner, R A.]] | + | |
| - | [[Category: Subirana, J A.]] | + | |
| - | [[Category: Valls, N.]] | + | |
| - | [[Category: Wright, G.]] | + | |
| - | [[Category: cobalt]]
| + | |
| - | [[Category: deoxyoligonucleotide]]
| + | |
| - | [[Category: variability]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:36:15 2008''
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