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| - | [[Image:1s2r.gif|left|200px]] | |
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| - | {{Structure
| + | ==A High Resolution Crystal Structure of [d(CGCAAATTTGCG)]2== |
| - | |PDB= 1s2r |SIZE=350|CAPTION= <scene name='initialview01'>1s2r</scene>, resolution 1.53Å
| + | <StructureSection load='1s2r' size='340' side='right'caption='[[1s2r]], [[Resolution|resolution]] 1.53Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene>
| + | <table><tr><td colspan='2'>[[1s2r]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S2R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S2R FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.53Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s2r OCA], [https://pdbe.org/1s2r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s2r RCSB], [https://www.ebi.ac.uk/pdbsum/1s2r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s2r ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s2r OCA], [http://www.ebi.ac.uk/pdbsum/1s2r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s2r RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''A High Resolution Crystal Structure of [d(CGCAAATTTGCG)]2'''
| + | [[Category: Howerton SB]] |
| - | | + | [[Category: Maehigashi T]] |
| - | | + | [[Category: Tannenbaum S]] |
| - | ==Overview==
| + | [[Category: Williams LD]] |
| - | The crystal structure of [d(CGCAAATTTGCG)]2 has been determined to 1.5 A resolution, representing the first high-resolution structure of this DNA fragment. The ion interactions are novel. A spermine molecule replaces a Mg2+ observed in analogous structures. Unlike lower-resolution structures, the minor groove is narrow and the major groove lacks extra Watson-Crick hydrogen bonds. In addition, a monolayer of solvent sites, including a "spine of hydration", is visible in the minor groove. The crystal of [d(CGCAAATTTGCG)]2 was grown from a solution containing spermine, magnesium, and lithium. The conformation recapitulates that of "monovalent-minus" DNA.
| + | [[Category: Woods KK]] |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1S2R is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S2R OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | High-resolution structure of an extended A-tract: [d(CGCAAATTTGCG)]2., Woods KK, Maehigashi T, Howerton SB, Sines CC, Tannenbaum S, Williams LD, J Am Chem Soc. 2004 Dec 1;126(47):15330-1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15563130 15563130]
| + | |
| - | [[Category: Protein complex]] | + | |
| - | [[Category: Howerton, S B.]] | + | |
| - | [[Category: Maehigashi, T.]] | + | |
| - | [[Category: Tannenbaum, S.]] | + | |
| - | [[Category: Williams, L D.]] | + | |
| - | [[Category: Woods, K K.]] | + | |
| - | [[Category: dodecamer]]
| + | |
| - | [[Category: minor groove hydration]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:36:32 2008''
| + | |
