1s7f

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[[Image:1s7f.gif|left|200px]]
 
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{{Structure
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==RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form I (apo)==
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|PDB= 1s7f |SIZE=350|CAPTION= <scene name='initialview01'>1s7f</scene>, resolution 2.0&Aring;
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<StructureSection load='1s7f' size='340' side='right'caption='[[1s7f]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>
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<table><tr><td colspan='2'>[[1s7f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S7F FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE= RimL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 Salmonella typhimurium LT2])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s7f OCA], [https://pdbe.org/1s7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s7f RCSB], [https://www.ebi.ac.uk/pdbsum/1s7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s7f ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1s7k|1S7K]], [[1s7l|1S7L]], [[1s7n|1S7N]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s7f OCA], [http://www.ebi.ac.uk/pdbsum/1s7f PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s7f RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q8ZPC0_SALTY Q8ZPC0_SALTY]
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== Evolutionary Conservation ==
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'''RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form I (apo)'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s7/1s7f_consurf.spt"</scriptWhenChecked>
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RimL is responsible for converting the prokaryotic ribosomal protein from L12 to L7 by acetylation of its N-terminal amino group. We demonstrate that purified RimL is capable of posttranslationally acetylating L12, exhibiting a V(max) of 21 min(-1). We have also determined the apostructure of RimL from Salmonella typhimurium and its complex with coenzyme A, revealing a homodimeric oligomer with structural similarity to other Gcn5-related N-acetyltransferase superfamily members. A large central trough located at the dimer interface provides sufficient room to bind both L12 N-terminal helices. Structural and biochemical analysis indicates that RimL proceeds by single-step transfer rather than a covalent-enzyme intermediate. This is the first structure of a Gcn5-related N-acetyltransferase family member with demonstrated activity toward a protein N(alpha)-amino group and is a first step toward understanding the molecular basis for N(alpha)acetylation and its function in cellular regulation.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1S7F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium_lt2 Salmonella typhimurium lt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7F OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s7f ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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A novel dimeric structure of the RimL Nalpha-acetyltransferase from Salmonella typhimurium., Vetting MW, de Carvalho LP, Roderick SL, Blanchard JS, J Biol Chem. 2005 Jun 10;280(23):22108-14. Epub 2005 Apr 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15817456 15817456]
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</StructureSection>
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[[Category: Salmonella typhimurium lt2]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
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[[Category: Blanchard, J S.]]
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[[Category: Blanchard JS]]
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[[Category: Carvalho, L P.de.]]
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[[Category: Roderick SL]]
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[[Category: Roderick, S L.]]
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[[Category: Vetting MW]]
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[[Category: Vetting, M W.]]
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[[Category: De Carvalho LP]]
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[[Category: acetyltransferase]]
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[[Category: alpha-n-protein acetyltransferase]]
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[[Category: coenzyme some]]
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[[Category: gnat]]
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[[Category: l7/l12]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:38:25 2008''
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Current revision

RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form I (apo)

PDB ID 1s7f

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