|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal structure of BoxB with malonate bound to the diiron center== | | ==Crystal structure of BoxB with malonate bound to the diiron center== |
| - | <StructureSection load='3pf7' size='340' side='right' caption='[[3pf7]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='3pf7' size='340' side='right'caption='[[3pf7]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3pf7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Azoev Azoev]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PF7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3pf7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aromatoleum_evansii Aromatoleum evansii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PF7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.903Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3per|3per]], [[3pm5|3pm5]], [[3q1g|3q1g]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">boxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=59406 AZOEV])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pf7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf7 OCA], [https://pdbe.org/3pf7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pf7 RCSB], [https://www.ebi.ac.uk/pdbsum/3pf7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pf7 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Benzoyl-CoA_2,3-dioxygenase Benzoyl-CoA 2,3-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.12.21 1.14.12.21] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pf7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf7 OCA], [http://pdbe.org/3pf7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pf7 RCSB], [http://www.ebi.ac.uk/pdbsum/3pf7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pf7 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BOXB_AZOEV BOXB_AZOEV]] The BoxA/BoxB complex catalyzes the aerobic reduction/oxygenation of the aromatic ring of benzoyl-CoA to form 2,3-dihydro-2,3-dihydroxybenzoyl-CoA. BoxB acts as the benzoyl-CoA oxygenase, after being reduced by the reductase component BoxA. BoxAB does not act on NADH or benzoate.<ref>PMID:15458418</ref> | + | [https://www.uniprot.org/uniprot/BOXB_AROEV BOXB_AROEV] The BoxA/BoxB complex catalyzes the aerobic reduction/oxygenation of the aromatic ring of benzoyl-CoA to form 2,3-epoxy-2,3-dihydrobenzoyl-CoA. BoxB acts as the benzoyl-CoA oxygenase, after being reduced by the reductase component BoxA. BoxAB does not act on NADH or benzoate.<ref>PMID:15458418</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The coenzyme A (CoA)-dependent aerobic benzoate metabolic pathway uses an unprecedented chemical strategy to overcome the high aromatic resonance energy by forming the non-aromatic 2,3-epoxybenzoyl-CoA. The crucial dearomatizing reaction is catalyzed by three enzymes, BoxABC, where BoxA is an NADPH-dependent reductase, BoxB is a benzoyl-CoA 2,3-epoxidase, and BoxC is an epoxide ring hydrolase. We characterized the key enzyme BoxB from Azoarcus evansii by structural and Mossbauer spectroscopic methods as a new member of class I diiron enzymes. Several family members were structurally studied with respect to the diiron center architecture, but no structure of an intact diiron enzyme with its natural substrate has been reported. X-ray structures between 1.9 and 2.5 A resolution were determined for BoxB in the diferric state and with bound substrate benzoyl-CoA in the reduced state. The substrate-bound reduced state is distinguished from the diferric state by increased iron-ligand distances and the absence of directly bridging groups between them. The position of benzoyl-CoA inside a 20 A long channel and the position of the phenyl ring relative to the diiron center are accurately defined. The C2 and C3 atoms of the phenyl ring are closer to one of the irons. Therefore, one oxygen of activated O(2) must be ligated predominantly to this proximate iron to be in a geometrically suitable position to attack the phenyl ring. Consistent with the observed iron/phenyl geometry, BoxB stereoselectively should form the 2S,3R-epoxide. We postulate a reaction cycle that allows a charge delocalization because of the phenyl ring and the electron-withdrawing CoA thioester.
| + | |
| - | | + | |
| - | Structure and Mechanism of the Diiron Benzoyl-Coenzyme A Epoxidase BoxB.,Rather LJ, Weinert T, Demmer U, Bill E, Ismail W, Fuchs G, Ermler U J Biol Chem. 2011 Aug 19;286(33):29241-8. Epub 2011 Jun 1. PMID:21632537<ref>PMID:21632537</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3pf7" style="background-color:#fffaf0;"></div>
| + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Azoev]] | + | [[Category: Aromatoleum evansii]] |
| - | [[Category: Benzoyl-CoA 2,3-dioxygenase]] | + | [[Category: Large Structures]] |
| - | [[Category: Ermler, U]] | + | [[Category: Ermler U]] |
| - | [[Category: Fuchs, G]] | + | [[Category: Fuchs G]] |
| - | [[Category: Rather, L J]] | + | [[Category: Rather LJ]] |
| - | [[Category: Weinert, T]] | + | [[Category: Weinert T]] |
| - | [[Category: Benzoyl coenzyme some]]
| + | |
| - | [[Category: Diiron center]]
| + | |
| - | [[Category: Epoxidase]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
