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| - | [[Image:1saq.gif|left|200px]] | |
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| - | {{Structure
| + | ==Crystal Structure of the RNA octamer GIC(GA)GCC== |
| - | |PDB= 1saq |SIZE=350|CAPTION= <scene name='initialview01'>1saq</scene>, resolution 2.7Å
| + | <StructureSection load='1saq' size='340' side='right'caption='[[1saq]], [[Resolution|resolution]] 2.70Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=I:INOSINIC+ACID'>I</scene>
| + | <table><tr><td colspan='2'>[[1saq]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SAQ FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1saq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1saq OCA], [https://pdbe.org/1saq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1saq RCSB], [https://www.ebi.ac.uk/pdbsum/1saq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1saq ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=[[1sa9|1SA9]]
| + | __TOC__ |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1saq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1saq OCA], [http://www.ebi.ac.uk/pdbsum/1saq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1saq RCSB]</span>
| + | </StructureSection> |
| - | }}
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Baeyens K]] |
| - | '''Crystal Structure of the RNA octamer GIC(GA)GCC'''
| + | [[Category: Holbrook SR]] |
| - | | + | [[Category: Jang SB]] |
| - | | + | [[Category: Jeong MS]] |
| - | ==Overview==
| + | [[Category: SantaLucia Jr J]] |
| - | The crystal structures of two RNA octamers, 5'-GGC(GA)GCC-3' and 5'-GIC(GA)GCC-3', have been determined from X-ray diffraction data to 2.8 and 2.7 A resolution, respectively. The RNA octamers crystallize in isomorphous unit cells containing two mispairs arranged in a self-complementary manner and one single strand in the asymmetric unit. The single strand pairs with another single strand related by crystallographic symmetry to form a third unique double helix. Tandem non-Watson-Crick G.A/A.G base pairs of the sheared type comprise an internal loop in the middle of each duplex. The NMR structure of this octameric RNA sequence is also known, allowing comparison of the variation between the six crystallographic duplexes and the solution structure. In the symmetric duplex of the octamer containing inosine, the sheared G.A pairs incorporate a bound water molecule. This duplex also binds one water molecule per strand in the minor groove adjacent to the G.A pairs.
| + | [[Category: Turner D]] |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1SAQ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAQ OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Structures of two RNA octamers containing tandem G.A base pairs., Jang SB, Baeyens K, Jeong MS, SantaLucia J Jr, Turner D, Holbrook SR, Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):829-35. Epub 2004, Apr 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15103128 15103128]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Baeyens, K.]] | + | |
| - | [[Category: Holbrook, S R.]] | + | |
| - | [[Category: Jang, S B.]] | + | |
| - | [[Category: Jeong, M S.]] | + | |
| - | [[Category: Jr, J SantaLucia.]] | + | |
| - | [[Category: Turner, D.]] | + | |
| - | [[Category: rna double helix]]
| + | |
| - | [[Category: rna tandem g-a base pair]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:39:31 2008''
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