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Epoxidase
From Proteopedia
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| - | <StructureSection load='3scf' size=' | + | <StructureSection load='3scf' size='350' side='right' caption='Hydroxypropylphosphonic acid epoxidase complex with fosfomycin, glycerol, NO and Fe+2 ion (PDB code [[3scf]])' scene='' pspeed='8'> |
== Function == | == Function == | ||
'''Hydroxypropylphosphonic acid epoxidase''' (HppE) is involved in the biosynthesis of the antibiotic fosfomycin<ref>PMID:16186494</ref>. HppE is a Zn+2/Fe+2-dependent enzyme. '''Halohydrin epoxidase''' (HHE) is involved in the degradation of halohydrins by the displacement of a halogen in halohydrins to produce the epoxide<ref>PMID:26422370</ref>. | '''Hydroxypropylphosphonic acid epoxidase''' (HppE) is involved in the biosynthesis of the antibiotic fosfomycin<ref>PMID:16186494</ref>. HppE is a Zn+2/Fe+2-dependent enzyme. '''Halohydrin epoxidase''' (HHE) is involved in the degradation of halohydrins by the displacement of a halogen in halohydrins to produce the epoxide<ref>PMID:26422370</ref>. | ||
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| - | == Disease == | ||
== Relevance == | == Relevance == | ||
| + | Fosfomycin is an antibiotic produced by some ''Streptomyces'' species. | ||
== Structural highlights == | == Structural highlights == | ||
| + | The <scene name='75/752206/Cv/6'>active site of HppE contains fosfomycin and Fe+2</scene>. The enzyme uses an induced-fit mechanism to protect high-energy iron-oxygen species formed during catalysis by moving a <scene name='75/752206/Cv/7'>β-hairpin termed cantilever</scene> to seal off the top portion of the active site<ref>PMID:21682308</ref>. <scene name='75/752206/Cv/8'>Fe coordination site</scene>. | ||
| - | + | ==3D structures of epoxidase== | |
| + | [[Epoxidase 3D structures]] | ||
</StructureSection> | </StructureSection> | ||
| - | ==3D structures of epoxidase== | ||
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| - | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
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| - | * 2-hydroxypropylphosphonic acid epoxidase | ||
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| - | **[[5u55]], [[5u5d]] – PsPsf4 + Mn + fosfomycin – ''Pseudomonas syringae''<br /> | ||
| - | **[[5u57]], [[5u58]] – PsPsf4 + Fe + fosfomycin <br /> | ||
| - | **[[4j1w]], [[4j1x]], [[3scf]], [[3scg]] – SwHppE + Fe + fosfomycin – ''Strepomyces wedmorensis'' <br /> | ||
| - | **[[1zz6]] – SwHppE <br /> | ||
| - | **[[2bnm]] – SwHppE + Zn <br /> | ||
| - | **[[1zz9]] – SwHppE + Fe <br /> | ||
| - | **[[1zzc]] – SwHppE + Co <br /> | ||
| - | **[[1zz7]], [[1zz8]] – SwHppE + Fe + fosfomycin <br /> | ||
| - | **[[3sch]], [[1zzb]] – SwHppE + Co + fosfomycin <br /> | ||
| - | **[[2bnn]], [[2bno]] – SwHppE + Zn + fosfomycin <br /> | ||
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| - | * Halohydrin epoxidase | ||
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| - | **[[5b12]] – CoHHE B (mutant) - ''Corynebacterium'' <br /> | ||
| - | **[[4zd9]], [[4zu3]] – CoHHE B <br /> | ||
| - | **[[4z9f]] – CoHHE A (mutant) <br /> | ||
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| - | * P450 epoxidase see [[Cytochrome P450]] | ||
| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
| + | [[Category:Topic Page]] | ||
Current revision
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References
- ↑ McLuskey K, Cameron S, Hammerschmidt F, Hunter WN. Structure and reactivity of hydroxypropylphosphonic acid epoxidase in fosfomycin biosynthesis by a cation- and flavin-dependent mechanism. Proc Natl Acad Sci U S A. 2005 Oct 4;102(40):14221-6. Epub 2005 Sep 26. PMID:16186494
- ↑ Watanabe F, Yu F, Ohtaki A, Yamanaka Y, Noguchi K, Yohda M, Odaka M. Crystal structures of halohydrin hydrogen-halide-lyases from Corynebacterium sp. N-1074. Proteins. 2015 Dec;83(12):2230-9. doi: 10.1002/prot.24938. Epub 2015 Oct 16. PMID:26422370 doi:http://dx.doi.org/10.1002/prot.24938
- ↑ Yun D, Dey M, Higgins LJ, Yan F, Liu HW, Drennan CL. Structural Basis of Regiospecificity of a Mononuclear Iron Enzyme in Antibiotic Fosfomycin Biosynthesis. J Am Chem Soc. 2011 Jun 30. PMID:21682308 doi:10.1021/ja2025728
