Mutations in Brca1 BRCT Domains
From Proteopedia
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| - | <StructureSection load='1T15_w_HybridPeptide.pdb' size=' | + | <StructureSection load='1T15_w_HybridPeptide.pdb' size='350' side='right' caption='Human BRCA1 (blue) complex with phosphoserine-containing BRCA1 interacting protein C-terminal helicase 1 (olive) (PDB code [[1t15]])' scene='75/752201/Cv/5' pspeed='8'> |
| - | Germline mutations in the BRCA1 tumor suppressor gene often result in a significant increase in susceptibility to breast and ovarian cancers. Although the molecular basis of their effects remains largely obscure, many mutations are known to target the highly conserved C-terminal BRCT repeats that function as a phosphoserine/phosphothreonine-binding module. We report the X-ray crystal structure at a resolution of 1.85 A of the BRCA1 tandem BRCT domains in complex with a phosphorylated peptide representing the minimal interacting region of the DEAH-box helicase BACH1. The structure reveals the determinants of this novel class of BRCA1 binding events. We show that a subset of disease-linked mutations act through specific disruption of phospho-dependent BRCA1 interactions rather than through gross structural perturbation of the tandem BRCT domains.<ref>PMID: 15133502</ref> | + | Germline mutations in the '''BRCA1 tumor suppressor''' gene often result in a significant increase in susceptibility to breast and ovarian cancers. Although the molecular basis of their effects remains largely obscure, many mutations are known to target the highly conserved C-terminal BRCT repeats that function as a phosphoserine/phosphothreonine-binding module. We report the X-ray crystal structure at a resolution of 1.85 A of the BRCA1 tandem BRCT domains in complex with a phosphorylated peptide representing the minimal interacting region of the DEAH-box helicase BACH1. The structure reveals the determinants of this novel class of BRCA1 binding events. We show that a subset of disease-linked mutations act through specific disruption of phospho-dependent BRCA1 interactions rather than through gross structural perturbation of the tandem BRCT domains.<ref>PMID: 15133502</ref> |
<scene name='75/752201/Cv/4'>Brca1 BRCT domains in complex with the phosphorylated interacting region from Bach1 Helicase</scene>. | <scene name='75/752201/Cv/4'>Brca1 BRCT domains in complex with the phosphorylated interacting region from Bach1 Helicase</scene>. | ||
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==Pathogenic mutations== | ==Pathogenic mutations== | ||
| - | *<scene name='75/752201/T1685a/ | + | *<scene name='75/752201/T1685a/4'>Mutation T1685A:</scene> |
<jmol> | <jmol> | ||
<jmolLink> | <jmolLink> | ||
| Line 46: | Line 46: | ||
</jmolLink> | </jmolLink> | ||
</jmol> between the two states. This mutation causes hydrogen bonds lost. | </jmol> between the two states. This mutation causes hydrogen bonds lost. | ||
| - | *<scene name='75/752201/T1685i/ | + | *<scene name='75/752201/T1685i/5'>Mutation T1685I:</scene> |
<jmol> | <jmol> | ||
<jmolLink> | <jmolLink> | ||
| Line 84: | Line 84: | ||
</jmolLink> | </jmolLink> | ||
</jmol> between the two states. This mutation causes hydrogen bonds lost. | </jmol> between the two states. This mutation causes hydrogen bonds lost. | ||
| - | *<scene name='75/752201/ | + | |
| + | *<scene name='75/752201/G1706e/4'>Mutation G1706E:</scene> | ||
<jmol> | <jmol> | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [ | + | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [GLY]1706;color selectionHalos green;selectionHalos on; |
</script> | </script> | ||
<text>Wild type </text> | <text>Wild type </text> | ||
| Line 97: | Line 98: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [ | + | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [GLU]1706;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutation. | <text>mutation. | ||
| Line 107: | Line 108: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation off; frame all; select [ | + | animation off; frame all; select [GLU]1706;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutated and wild type | <text>mutated and wild type | ||
| Line 116: | Line 117: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame play; select [ | + | animation on; animation mode loop; frame play; select [GLU]1706;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>animation | <text>animation | ||
</text> | </text> | ||
</jmolLink> | </jmolLink> | ||
| - | </jmol> between the two states. This mutation causes | + | </jmol> between the two states. This mutation causes overpacking, and forms new hydrogen bonds. |
| - | *<scene name='75/752201/ | + | |
| + | *<scene name='75/752201/S1715r/4'>Mutation S1715R:</scene> | ||
<jmol> | <jmol> | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [ | + | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [SER]1715;color selectionHalos green;selectionHalos on; |
</script> | </script> | ||
<text>Wild type </text> | <text>Wild type </text> | ||
| Line 135: | Line 137: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [ | + | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [ARG]1715;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutation. | <text>mutation. | ||
| Line 145: | Line 147: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation off; frame all; select [ | + | animation off; frame all; select [ARG]1715;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutated and wild type | <text>mutated and wild type | ||
| Line 154: | Line 156: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame play; select [ | + | animation on; animation mode loop; frame play; select [ARG]1715;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>animation | <text>animation | ||
</text> | </text> | ||
</jmolLink> | </jmolLink> | ||
| - | </jmol> between the two states. This mutation causes | + | </jmol> between the two states. This mutation causes hydrogen bonds lost, overpacking. |
| - | *<scene name='75/752201/ | + | *<scene name='75/752201/G1738r/4'>Mutation G1738R:</scene> |
<jmol> | <jmol> | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [ | + | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [GLY]1738;color selectionHalos green;selectionHalos on; |
</script> | </script> | ||
<text>Wild type </text> | <text>Wild type </text> | ||
| Line 173: | Line 175: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [ | + | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [ARG]1738;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutation. | <text>mutation. | ||
| Line 183: | Line 185: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation off; frame all; select [ | + | animation off; frame all; select [ARG]1738;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutated and wild type | <text>mutated and wild type | ||
| Line 192: | Line 194: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame play; select [ | + | animation on; animation mode loop; frame play; select [ARG]1738;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>animation | <text>animation | ||
</text> | </text> | ||
</jmolLink> | </jmolLink> | ||
| - | </jmol> between the two states. This mutation causes overpacking | + | </jmol> between the two states. This mutation causes overpacking. |
| - | *<scene name='75/752201/ | + | *<scene name='75/752201/L1764p/5'>Mutation L1764P:</scene> |
<jmol> | <jmol> | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [ | + | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [LEU]1764;color selectionHalos green;selectionHalos on; |
</script> | </script> | ||
<text>Wild type </text> | <text>Wild type </text> | ||
| Line 211: | Line 213: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [ | + | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [PRO]1764;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutation. | <text>mutation. | ||
| Line 221: | Line 223: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation off; frame all; select [ | + | animation off; frame all; select [PRO]1764;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutated and wild type | <text>mutated and wild type | ||
| Line 230: | Line 232: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame play; select [ | + | animation on; animation mode loop; frame play; select [PRO]1764;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>animation | <text>animation | ||
</text> | </text> | ||
</jmolLink> | </jmolLink> | ||
| - | </jmol> between the two states. This mutation causes | + | </jmol> between the two states. This mutation causes overpacking. |
| - | *<scene name='75/752201/ | + | *<scene name='75/752201/I1766s/2'>Mutation I1766S:</scene> |
<jmol> | <jmol> | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [ | + | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [ILE]1766;color selectionHalos green;selectionHalos on; |
</script> | </script> | ||
<text>Wild type </text> | <text>Wild type </text> | ||
| Line 249: | Line 251: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [ | + | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [SER]1766;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutation. | <text>mutation. | ||
| Line 259: | Line 261: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation off; frame all; select [ | + | animation off; frame all; select [SER]1766;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutated and wild type | <text>mutated and wild type | ||
| Line 268: | Line 270: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame play; select [ | + | animation on; animation mode loop; frame play; select [SER]1766;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>animation | <text>animation | ||
</text> | </text> | ||
</jmolLink> | </jmolLink> | ||
| - | </jmol> between the two states. This mutation | + | </jmol> between the two states. This mutation probably decreased hydrophobic interaction. |
| - | *<scene name='75/752201/ | + | |
| + | *<scene name='75/752201/C1787s/2'>Mutation C1787S:</scene> | ||
<jmol> | <jmol> | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [ | + | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [CYS]1787;color selectionHalos green;selectionHalos on; |
</script> | </script> | ||
<text>Wild type </text> | <text>Wild type </text> | ||
| Line 287: | Line 290: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [ | + | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [SER]1787;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutation. | <text>mutation. | ||
| Line 297: | Line 300: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation off; frame all; select [ | + | animation off; frame all; select [SER]1787;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutated and wild type | <text>mutated and wild type | ||
| Line 306: | Line 309: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame play; select [ | + | animation on; animation mode loop; frame play; select [SER]1787;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>animation | <text>animation | ||
</text> | </text> | ||
</jmolLink> | </jmolLink> | ||
| - | </jmol> between the two states | + | </jmol> between the two states. |
| - | *<scene name='75/752201/ | + | *<scene name='75/752201/G1788v/4'>Mutation G1788V:</scene> |
<jmol> | <jmol> | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [ | + | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [GLY]1788;color selectionHalos green;selectionHalos on; |
</script> | </script> | ||
<text>Wild type </text> | <text>Wild type </text> | ||
| Line 325: | Line 328: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [ | + | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [VAL]1788;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutation. | <text>mutation. | ||
| Line 335: | Line 338: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation off; frame all; select [ | + | animation off; frame all; select [VAL]1788;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutated and wild type | <text>mutated and wild type | ||
| Line 344: | Line 347: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame play; select [ | + | animation on; animation mode loop; frame play; select [VAL]1788;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>animation | <text>animation | ||
</text> | </text> | ||
</jmolLink> | </jmolLink> | ||
| - | </jmol> between the two states. This mutation | + | </jmol> between the two states. This mutation causes overpacking. |
| - | *<scene name='75/752201/ | + | *<scene name='75/752201/V1838e/1'>Mutation V1838E:</scene> |
<jmol> | <jmol> | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [ | + | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [VAL]1838;color selectionHalos green;selectionHalos on; |
</script> | </script> | ||
| - | <text>Wild type | + | <text>Wild type </text> |
</jmolLink> | </jmolLink> | ||
</jmol> | </jmol> | ||
| Line 363: | Line 366: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [ | + | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [GLU]1838;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
| - | <text>mutation | + | <text>mutation. |
</text> | </text> | ||
</jmolLink> | </jmolLink> | ||
| Line 373: | Line 376: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation off; frame all; select [ | + | animation off; frame all; select [GLU]1838;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutated and wild type | <text>mutated and wild type | ||
| Line 382: | Line 385: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame play; select [ | + | animation on; animation mode loop; frame play; select [GLU]1838;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>animation | <text>animation | ||
</text> | </text> | ||
</jmolLink> | </jmolLink> | ||
| - | </jmol> between the two states. | + | </jmol> between the two states. This mutation causes overpacking. |
| - | + | ||
| - | *<scene name='75/752201/ | + | === Very severe pathogenic mutations === |
| + | *<scene name='75/752201/R1899w/4'>Mutation R1699W:</scene> | ||
<jmol> | <jmol> | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [ | + | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [ARG]1699;color selectionHalos green;selectionHalos on; |
</script> | </script> | ||
<text>Wild type </text> | <text>Wild type </text> | ||
| Line 402: | Line 406: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [ | + | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [TRP]1699;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutation. | <text>mutation. | ||
| Line 412: | Line 416: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation off; frame all; select [ | + | animation off; frame all; select [TRP]1699;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutated and wild type | <text>mutated and wild type | ||
| Line 421: | Line 425: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame play; select [ | + | animation on; animation mode loop; frame play; select [TRP]1699;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>animation | <text>animation | ||
</text> | </text> | ||
</jmolLink> | </jmolLink> | ||
| - | </jmol> between the two states. | + | </jmol> between the two states. This mutation causes saltbridges lost, hydrogen bonds lost, overpacking, clashes; '''interaction lost with BRCA1 interacting protein C-terminal helicase 1'''. |
| - | *<scene name='75/752201/ | + | *<scene name='75/752201/A1708e/4'>Mutation A1708E:</scene> |
<jmol> | <jmol> | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [ | + | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [ALA]1708;color selectionHalos green;selectionHalos on; |
</script> | </script> | ||
<text>Wild type </text> | <text>Wild type </text> | ||
| Line 440: | Line 444: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [ | + | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [GLU]1708;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutation. | <text>mutation. | ||
| Line 450: | Line 454: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation off; frame all; select [ | + | animation off; frame all; select [GLU]1708;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutated and wild type | <text>mutated and wild type | ||
| Line 459: | Line 463: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame play; select [ | + | animation on; animation mode loop; frame play; select [GLU]1708;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>animation | <text>animation | ||
</text> | </text> | ||
</jmolLink> | </jmolLink> | ||
| - | </jmol> between the two states. | + | </jmol> between the two states. This mutation causes overpacking, and forms new hydrogen bond. |
| - | *<scene name='75/752201/ | + | *<scene name='75/752201/M1775r/10'>Mutation M1775R:</scene> |
<jmol> | <jmol> | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [ | + | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [MET]1775;color selectionHalos green;selectionHalos on; |
</script> | </script> | ||
| - | <text>Wild type </text> | + | <text>Wild type</text> |
</jmolLink> | </jmolLink> | ||
</jmol> | </jmol> | ||
| Line 478: | Line 482: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [ | + | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [ARG]1775;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutation. | <text>mutation. | ||
| Line 488: | Line 492: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation off; frame all; select [ | + | animation off; frame all; select [ARG]1775;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutated and wild type | <text>mutated and wild type | ||
| Line 497: | Line 501: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame play; select [ | + | animation on; animation mode loop; frame play; select [ARG]1775;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>animation | <text>animation | ||
</text> | </text> | ||
</jmolLink> | </jmolLink> | ||
| - | </jmol> between the two states. This mutation causes | + | </jmol> between the two states. This mutation causes interference with phosphorylated interacting region from Bach1 Helicase. |
| - | *<scene name='75/752201/ | + | *<scene name='75/752201/M1775k/5'>Mutation M1775K:</scene> |
<jmol> | <jmol> | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [ | + | animation on; animation mode loop; frame 1 1 play; animation off; frame 1; select [MET]1775;color selectionHalos green;selectionHalos on; |
</script> | </script> | ||
<text>Wild type </text> | <text>Wild type </text> | ||
| Line 516: | Line 520: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [ | + | animation on; animation mode loop; frame 2 2 play; animation off; frame 2; select [LYS]1775;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutation. | <text>mutation. | ||
| Line 526: | Line 530: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation off; frame all; select [ | + | animation off; frame all; select [LYS]1775;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>mutated and wild type | <text>mutated and wild type | ||
| Line 535: | Line 539: | ||
<jmolLink> | <jmolLink> | ||
<script> | <script> | ||
| - | animation on; animation mode loop; frame play; select [ | + | animation on; animation mode loop; frame play; select [LYS]1775;color selectionHalos red;selectionHalos on; |
</script> | </script> | ||
<text>animation | <text>animation | ||
</text> | </text> | ||
</jmolLink> | </jmolLink> | ||
| - | </jmol> between the two states. This mutation causes | + | </jmol> between the two states. This mutation causes interference with phosphorylated interacting region from Bach1 Helicase. |
==Benign mutations== | ==Benign mutations== | ||
Current revision
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References
- ↑ Clapperton JA, Manke IA, Lowery DM, Ho T, Haire LF, Yaffe MB, Smerdon SJ. Structure and mechanism of BRCA1 BRCT domain recognition of phosphorylated BACH1 with implications for cancer. Nat Struct Mol Biol. 2004 Jun;11(6):512-8. Epub 2004 May 9. PMID:15133502 doi:10.1038/nsmb775
