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| | ==L-selectin lectin and EGF domains== | | ==L-selectin lectin and EGF domains== |
| - | <StructureSection load='3cfw' size='340' side='right' caption='[[3cfw]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='3cfw' size='340' side='right'caption='[[3cfw]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3cfw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CFW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3cfw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CFW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CFW FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1esl|1esl]], [[1g1q|1g1q]], [[1g1r|1g1r]], [[1g1s|1g1s]], [[1g1t|1g1t]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cfw OCA], [http://pdbe.org/3cfw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cfw RCSB], [http://www.ebi.ac.uk/pdbsum/3cfw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cfw ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cfw OCA], [https://pdbe.org/3cfw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cfw RCSB], [https://www.ebi.ac.uk/pdbsum/3cfw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cfw ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LYAM1_HUMAN LYAM1_HUMAN]] Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.<ref>PMID:12403782</ref> | + | [https://www.uniprot.org/uniprot/LYAM1_HUMAN LYAM1_HUMAN] Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.<ref>PMID:12403782</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> | | Check<jmol> |
| | <jmolCheckbox> | | <jmolCheckbox> |
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/3cfw_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/3cfw_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Mather, T]] | + | [[Category: Homo sapiens]] |
| - | [[Category: McEver, R P]] | + | [[Category: Large Structures]] |
| - | [[Category: Mehta, P]] | + | [[Category: Mather T]] |
| - | [[Category: Oganesyan, V]] | + | [[Category: McEver RP]] |
| - | [[Category: Terzyan, S]] | + | [[Category: Mehta P]] |
| - | [[Category: Cell adhesion]] | + | [[Category: Oganesyan V]] |
| - | [[Category: Egf]] | + | [[Category: Terzyan S]] |
| - | [[Category: Egf-like domain]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: L-selectin]]
| + | |
| - | [[Category: Lectin]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Sushi]]
| + | |
| - | [[Category: Transmembrane]]
| + | |
| Structural highlights
Function
LYAM1_HUMAN Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Selectin interactions with fucosylated glycan ligands mediate leukocyte rolling in the vasculature under shear forces. Crystal structures of P- and E-selectin suggest a two-state model in which ligand binding to the lectin domain closes loop 83-89 around the Ca2+ coordination site, enabling Glu-88 to engage Ca2+ and fucose. This triggers further allostery that opens the lectin/EGF domain hinge. The model posits that force accelerates transition from the bent (low affinity) to the extended (high affinity) state. However, transition intermediates have not been described, and the role of Glu-88 in force-assisted allostery has not been examined. Here we report the structure of the lectin and EGF domains of L-selectin bound to a fucose mimetic; that is, a terminal mannose on an N-glycan attached to a symmetry-related molecule. The structure is a transition intermediate where loop 83-89 closes to engage Ca2+ and mannose without triggering allostery that opens the lectin/EGF domain hinge. We used three complementary assays to compare ligand binding to WT selectins and to E88D selectins that replaced Glu-88 with Asp. Soluble P-selectinE88D bound with an approximately 9-fold lower affinity to PSGL-1, a physiological ligand, due to faster dissociation. Adhesion frequency experiments with a biomembrane force probe could not detect interactions of P-selectinE88D with PSGL-1. Cells expressing transmembrane P-selectinE88D or L-selectinE88D detached from immobilized ligands immediately after initiating flow. Cells expressing E-selectinE88D rolled but detached faster. Our data support a two-state model for selectins in which Glu-88 must engage ligand to trigger allostery that stabilizes the high affinity state under force.
Glycan Bound to the Selectin Low Affinity State Engages Glu-88 to Stabilize the High Affinity State under Force.,Mehta-D'souza P, Klopocki AG, Oganesyan V, Terzyan S, Mather T, Li Z, Panicker SR, Zhu C, McEver RP J Biol Chem. 2017 Feb 10;292(6):2510-2518. doi: 10.1074/jbc.M116.767186. Epub, 2016 Dec 23. PMID:28011641[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Bernimoulin MP, Zeng XL, Abbal C, Giraud S, Martinez M, Michielin O, Schapira M, Spertini O. Molecular basis of leukocyte rolling on PSGL-1. Predominant role of core-2 O-glycans and of tyrosine sulfate residue 51. J Biol Chem. 2003 Jan 3;278(1):37-47. Epub 2002 Oct 25. PMID:12403782 doi:10.1074/jbc.M204360200
- ↑ Mehta-D'souza P, Klopocki AG, Oganesyan V, Terzyan S, Mather T, Li Z, Panicker SR, Zhu C, McEver RP. Glycan Bound to the Selectin Low Affinity State Engages Glu-88 to Stabilize the High Affinity State under Force. J Biol Chem. 2017 Feb 10;292(6):2510-2518. doi: 10.1074/jbc.M116.767186. Epub, 2016 Dec 23. PMID:28011641 doi:http://dx.doi.org/10.1074/jbc.M116.767186
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