4qyj

<StructureSection load='4qyj' size='340' side='right' caption='[[4qyj]], [[Resolution|resolution]] 2.83&Aring;' scene=''>

<table><tr><td colspan='2'>[[4qyj]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QYJ FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qyj OCA], [http://pdbe.org/4qyj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qyj RCSB], [http://www.ebi.ac.uk/pdbsum/4qyj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qyj ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Phenylacetaldehyde dehydrogenase catalyzes the NAD+-dependent oxidation of phenylactealdehyde to phenylacetic acid in the styrene catabolic and detoxification pathway of Pseudomonas putida (S12). Here we report the structure and mechanistic properties of the N-terminally histidine-tagged enzyme, NPADH. The 2.83 A X-ray crystal structure is similar in fold to sheep liver cytosolic aldehyde dehydrogenase (ALDH1), but has unique set of intersubunit interactions and active site tunnel for substrate entrance. In solution, NPADH occurs as 227 kDa homotetramer. It follows a sequential reaction mechanism in which NAD+ serves as both the leading substrate and homotropic allosteric activator. In the absence of styrene monooxygenase reductase, which regenerates NAD+ from NADH in the first step of styrene catabolism, NPADH is inhibited by a ternary complex involving NADH, product, and phenylacetaldehyde, substrate. Each oligomerization domain of NPADH contains a six-residue insertion that extends this loop over the substrate entrance tunnel of a neighboring subunit, thereby obstructing the active site of the adjacent subunit. This feature could be an important factor in the homotropic activation and product inhibition mechanisms. Compared to ALDH1, the substrate channel of NPADH is narrower and lined with more aromatic residues, suggesting a means for enhancing substrate specificity.

 

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== References ==

[[Category: Crabo, A G]]

[[Category: Gassner, G T]]

[[Category: Sazinsky, M H]]

[[Category: Aldehyde dehydrogenase]]

[[Category: Oxidoreductase]]