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| - | [[Image:1si9.jpg|left|200px]] | |
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| - | {{Structure
| + | ==Boiling stable protein isolated from Populus tremula== |
| - | |PDB= 1si9 |SIZE=350|CAPTION= <scene name='initialview01'>1si9</scene>, resolution 2.27Å
| + | <StructureSection load='1si9' size='340' side='right'caption='[[1si9]], [[Resolution|resolution]] 2.27Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | + | <table><tr><td colspan='2'>[[1si9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_tremula Populus tremula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SI9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SI9 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1si9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1si9 OCA], [https://pdbe.org/1si9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1si9 RCSB], [https://www.ebi.ac.uk/pdbsum/1si9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1si9 ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1si9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1si9 OCA], [http://www.ebi.ac.uk/pdbsum/1si9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1si9 RCSB]</span>
| + | == Function == |
| - | }}
| + | [https://www.uniprot.org/uniprot/Q9AR79_POPTN Q9AR79_POPTN] |
| - | | + | == Evolutionary Conservation == |
| - | '''Boiling stable protein isolated from Populus tremula'''
| + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | | + | Check<jmol> |
| - | | + | <jmolCheckbox> |
| - | ==Overview== | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/si/1si9_consurf.spt"</scriptWhenChecked> |
| - | We previously reported on a new boiling stable protein isolated from aspen plants (Populus tremula), which we named SP1. SP1 is a stress-related protein with no significant sequence homology to other stress-related proteins. It is a 108-amino-acid hydrophilic polypeptide with a molecular mass of 12.4 kDa (Wang, W. X., Pelah, D., Alergand, T., Shoseyov, O., and Altman, A. (2002) Plant Physiol. 130, 865-875) and is found in an oligomeric form. Preliminary electron microscopy studies and matrix-assisted laser desorption ionization time-of-flight mass spectrometry experiments showed that SP1 is a dodecamer composed of two stacking hexamers. We performed a SDS-PAGE analysis, a differential scanning calorimetric study, and crystal structure determination to further characterize SP1. SDS-PAGE indicated a spontaneous assembly of SP1 to one stable oligomeric form, a dodecamer. Differential scanning calorimetric showed that SP1 has high thermostability i.e. Tm of 107 degrees C (at pH 7.8). The crystal structure of SP1 was initially determined to 2.4 A resolution by multi-wavelength anomalous dispersion method from a crystal belonging to the space group I422. The phases were extended to 1.8 A resolution using data from a different crystal form (P21). The final refined molecule includes 106 of the 108 residues and 132 water molecules (on average for each chain). The R-free is 20.1%. The crystal structure indicated that the SP1 molecule has a ferredoxin-like fold. Strong interactions between each two molecules create a stable dimer. Six dimers associate to form a ring-like-shaped dodecamer strongly resembling the particle visualized in the electron microscopy studies. No structural similarity was found between the crystal structure of SP1 and the crystal structure of other stress-related proteins such as small heat shock proteins, whose structure has been already determined. This structural study further supports our previous report that SP1 may represent a new family of stress-related proteins with high thermostability and oligomerization.
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | | + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | ==About this Structure== | + | </jmolCheckbox> |
| - | 1SI9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Populus_tremula Populus tremula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SI9 OCA].
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1si9 ConSurf]. |
| - | | + | <div style="clear:both"></div> |
| - | ==Reference==
| + | __TOC__ |
| - | The structural basis of the thermostability of SP1, a novel plant (Populus tremula) boiling stable protein., Dgany O, Gonzalez A, Sofer O, Wang W, Zolotnitsky G, Wolf A, Shoham Y, Altman A, Wolf SG, Shoseyov O, Almog O, J Biol Chem. 2004 Dec 3;279(49):51516-23. Epub 2004 Sep 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15371455 15371455]
| + | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Populus tremula]] | | [[Category: Populus tremula]] |
| - | [[Category: Single protein]]
| + | [[Category: Almog O]] |
| - | [[Category: Almog, O.]] | + | [[Category: Dgany O]] |
| - | [[Category: Dgany, O.]] | + | [[Category: Gonzales A]] |
| - | [[Category: Gonzales, A.]] | + | [[Category: Shoseyov O]] |
| - | [[Category: Shoseyov, O.]] | + | [[Category: Sofer O]] |
| - | [[Category: Sofer, O.]] | + | [[Category: Wolf SG]] |
| - | [[Category: Wolf, S G.]] | + | |
| - | [[Category: boiling-soluble]]
| + | |
| - | [[Category: heat shock responsive]]
| + | |
| - | [[Category: stress]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:42:25 2008''
| + | |
