1sjw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:31, 14 February 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1sjw.gif|left|200px]]
 
-
{{Structure
+
==Structure of polyketide cyclase SnoaL==
-
|PDB= 1sjw |SIZE=350|CAPTION= <scene name='initialview01'>1sjw</scene>, resolution 1.35&Aring;
+
<StructureSection load='1sjw' size='340' side='right'caption='[[1sjw]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=NGV:METHYL+5,7-DIHYDROXY-2-METHYL-4,6,11-TRIOXO-3,4,6,11-TETRAHYDROTETRACENE-1-CARBOXYLATE'>NGV</scene>
+
<table><tr><td colspan='2'>[[1sjw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SJW FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
-
|GENE= SnoaL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 Streptomyces nogalater])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NGV:METHYL+5,7-DIHYDROXY-2-METHYL-4,6,11-TRIOXO-3,4,6,11-TETRAHYDROTETRACENE-1-CARBOXYLATE'>NGV</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjw OCA], [https://pdbe.org/1sjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sjw RCSB], [https://www.ebi.ac.uk/pdbsum/1sjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sjw ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjw OCA], [http://www.ebi.ac.uk/pdbsum/1sjw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sjw RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/SNOAL_STRNO SNOAL_STRNO] Involved in the biosynthesis of the aromatic polyketide antibiotic nogalamycin. Catalyzes the formation of nogalaviketone from nogalonic acid methyl ester (NAME), the last ring-closure step in the biosynthesis of nogalamycin.<ref>PMID:10639368</ref> <ref>PMID:15071504</ref>
-
 
+
== Evolutionary Conservation ==
-
'''Structure of polyketide cyclase SnoaL'''
+
[[Image:Consurf_key_small.gif|200px|right]]
-
 
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==Overview==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sj/1sjw_consurf.spt"</scriptWhenChecked>
-
SnoaL belongs to a family of small polyketide cyclases, which catalyse ring closure steps in the biosynthesis of polyketide antibiotics produced in Streptomyces. Several of these antibiotics are among the most used anti-cancer drugs currently in use. The crystal structure of SnoaL, involved in nogalamycin biosynthesis, with a bound product, has been determined to 1.35 A resolution. The fold of the subunit can be described as a distorted alpha+beta barrel, and the ligand is bound in the hydrophobic interior of the barrel. The 3D structure and site-directed mutagenesis experiments reveal that the mechanism of the intramolecular aldol condensation catalysed by SnoaL is different from that of the classical aldolases, which employ covalent Schiff base formation or a metal ion cofactor. The invariant residue Asp121 acts as an acid/base catalyst during the reaction. Stabilisation of the enol(ate) intermediate is mainly achieved by the delocalisation of the electron pair over the extended pi system of the substrate. These polyketide cyclases thus form of family of enzymes with a unique catalytic strategy for aldol condensation.
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==About this Structure==
+
</jmolCheckbox>
-
1SJW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJW OCA].
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sjw ConSurf].
-
 
+
<div style="clear:both"></div>
-
==Reference==
+
== References ==
-
Structure of the polyketide cyclase SnoaL reveals a novel mechanism for enzymatic aldol condensation., Sultana A, Kallio P, Jansson A, Wang JS, Niemi J, Mantsala P, Schneider G, EMBO J. 2004 May 5;23(9):1911-21. Epub 2004 Apr 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15071504 15071504]
+
<references/>
-
[[Category: Single protein]]
+
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Streptomyces nogalater]]
[[Category: Streptomyces nogalater]]
-
[[Category: Jansson, A.]]
+
[[Category: Jansson A]]
-
[[Category: Kallio, P.]]
+
[[Category: Kallio P]]
-
[[Category: Mantsala, P.]]
+
[[Category: Mantsala P]]
-
[[Category: Neimi, J.]]
+
[[Category: Neimi J]]
-
[[Category: SPINE, Structural Proteomics in Europe.]]
+
[[Category: Schneider G]]
-
[[Category: Schneider, G.]]
+
[[Category: Sultana A]]
-
[[Category: Sultana, A.]]
+
[[Category: Wang JS]]
-
[[Category: Wang, J S.]]
+
-
[[Category: aldol condensation]]
+
-
[[Category: anthracycline]]
+
-
[[Category: crystal structure]]
+
-
[[Category: nogalamycin]]
+
-
[[Category: snoal]]
+
-
[[Category: spine]]
+
-
[[Category: structural genomic]]
+
-
[[Category: structural proteomics in europe]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:43:05 2008''
+

Current revision

Structure of polyketide cyclase SnoaL

PDB ID 1sjw

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1sjw"
Personal tools