1gvz

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[[Image:1gvz.gif|left|200px]]<br />
 
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<applet load="1gvz" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gvz, resolution 1.42&Aring;" />
 
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'''PROSTATE SPECIFIC ANTIGEN (PSA) FROM STALLION SEMINAL PLASMA'''<br />
 
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==Overview==
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==Prostate Specific Antigen (PSA) from stallion seminal plasma==
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Prostate-specific kallikrein, a member of the gene family of serine, proteases, was initially discovered in semen and is the most useful serum, marker for prostate cancer diagnosis and prognosis. We report the crystal, structure at 1.42A resolution of horse prostate kallikrein (HPK). This is, the first structure of a serine protease purified from seminal plasma. HPK, shares extensive sequence homology with human prostate-specific antigen, (PSA), including a predicted chymotrypsin-like specificity, as suggested, by the presence of a serine residue at position S1 of the specificity, pocket. In contrast to other kallikreins, HPK shows a structurally, distinct specificity pocket. Its entrance is blocked by the kallikrein, loop, suggesting a possible protective or substrate-selective role for, this loop. The HPK structure seems to be in an inactivated state and, further processing might be required to allow the binding of substrate, molecules. Crystal soaking experiments revealed a binding site for Zn(2+), and Hg(2+), two known PSA inhibitors.
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<StructureSection load='1gvz' size='340' side='right'caption='[[1gvz]], [[Resolution|resolution]] 1.42&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gvz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GVZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GVZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.42&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gvz OCA], [https://pdbe.org/1gvz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gvz RCSB], [https://www.ebi.ac.uk/pdbsum/1gvz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gvz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KLK2_HORSE KLK2_HORSE] Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gv/1gvz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gvz ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1GVZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus] with ACT and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVZ OCA].
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*[[Kallikrein 3D structures|Kallikrein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of a prostate kallikrein isolated from stallion seminal plasma: a homologue of human PSA., Carvalho AL, Sanz L, Barettino D, Romero A, Calvete JJ, Romao MJ, J Mol Biol. 2002 Sep 13;322(2):325-37. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12217694 12217694]
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[[Category: Equus caballus]]
[[Category: Equus caballus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Barettino, D.]]
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[[Category: Barettino D]]
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[[Category: Calvete, J.J.]]
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[[Category: Calvete JJ]]
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[[Category: Carvalho, A.L.]]
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[[Category: Carvalho AL]]
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[[Category: Romao, M.J.]]
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[[Category: Romao MJ]]
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[[Category: Romero, A.]]
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[[Category: Romero A]]
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[[Category: Sanz, L.]]
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[[Category: Sanz L]]
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[[Category: ACT]]
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[[Category: GOL]]
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[[Category: kallikrein]]
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[[Category: prostate specific antigen]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 16:20:52 2007''
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Current revision

Prostate Specific Antigen (PSA) from stallion seminal plasma

PDB ID 1gvz

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