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5mzx
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the decarboxylase AibA/AibB in complex with 4'-diphospho pantetheine== | |
| + | <StructureSection load='5mzx' size='340' side='right'caption='[[5mzx]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5mzx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxococcus_xanthus_DK_1622 Myxococcus xanthus DK 1622]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MZX FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4PS:4-DIPHOSPHO+PANTETHEINE'>4PS</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mzx OCA], [https://pdbe.org/5mzx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mzx RCSB], [https://www.ebi.ac.uk/pdbsum/5mzx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mzx ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q1D4I4_MYXXD Q1D4I4_MYXXD] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Isovaleryl coenzyme A (IV-CoA) is an important precursor for iso-fatty acids and lipids. It acts in the development of myxobacteria, which can produce this compound from acetyl-CoA through alternative IV-CoA biosynthesis (aib). A central reaction of aib is catalyzed by AibA/AibB, which acts as a cofactor-free decarboxylase despite belonging to the family of CoA-transferases. We developed an efficient expression system for AibA/AibB that allowed the determination of high-resolution crystal structures in complex with different ligands. Through mutational studies, we show that an active-site cysteine previously proposed to be involved in decarboxylation is not required for activity. Instead, AibA/AibB seems to induce an intramolecular decarboxylation by binding its substrate in a hydrophobic cavity and forcing it into a bent conformation. Our study opens opportunities for synthetic biology studies, since AibA/AibB may be suitable for the production of isobutene, a precursor of biofuels and chemicals. | ||
| - | + | AibA/AibB Induces an Intramolecular Decarboxylation in Isovalerate Biosynthesis by Myxococcus xanthus.,Bock T, Luxenburger E, Hoffmann J, Schutza V, Feiler C, Muller R, Blankenfeldt W Angew Chem Int Ed Engl. 2017 Aug 7;56(33):9986-9989. doi: 10.1002/anie.201701992., Epub 2017 May 16. PMID:28508504<ref>PMID:28508504</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5mzx" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Myxococcus xanthus DK 1622]] | ||
| + | [[Category: Blankenfeldt W]] | ||
| + | [[Category: Bock T]] | ||
| + | [[Category: Feiler C]] | ||
| + | [[Category: Hoffmann J]] | ||
| + | [[Category: Luxenburger E]] | ||
| + | [[Category: Mueller R]] | ||
| + | [[Category: Schuetza V]] | ||
Current revision
Crystal structure of the decarboxylase AibA/AibB in complex with 4'-diphospho pantetheine
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