1sp9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:33, 1 May 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1sp9.jpg|left|200px]]
 
-
{{Structure
+
==4-Hydroxyphenylpyruvate Dioxygenase==
-
|PDB= 1sp9 |SIZE=350|CAPTION= <scene name='initialview01'>1sp9</scene>, resolution 3.&Aring;
+
<StructureSection load='1sp9' size='340' side='right'caption='[[1sp9]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>
+
<table><tr><td colspan='2'>[[1sp9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SP9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SP9 FirstGlance]. <br>
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/4-hydroxyphenylpyruvate_dioxygenase 4-hydroxyphenylpyruvate dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.27 1.13.11.27] </span>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
-
|GENE= HPD, AT1G06570, F12K11.9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sp9 OCA], [https://pdbe.org/1sp9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sp9 RCSB], [https://www.ebi.ac.uk/pdbsum/1sp9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sp9 ProSAT]</span></td></tr>
-
|RELATEDENTRY=[[1sp8|1SP8]]
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sp9 OCA], [http://www.ebi.ac.uk/pdbsum/1sp9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sp9 RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/HPPD_ARATH HPPD_ARATH]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sp/1sp9_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sp9 ConSurf].
 +
<div style="clear:both"></div>
-
'''4-Hydroxyphenylpyruvate Dioxygenase'''
+
==See Also==
-
 
+
*[[Dioxygenase 3D structures|Dioxygenase 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
The transformation of 4-hydroxyphenylpyruvate to homogentisate, catalyzed by 4-hydroxyphenylpyruvate dioxygenase (HPPD), plays an important role in degrading aromatic amino acids. As the reaction product homogentisate serves as aromatic precursor for prenylquinone synthesis in plants, the enzyme is an interesting target for herbicides. In this study we report the first x-ray structures of the plant HPPDs of Zea mays and Arabidopsis in their substrate-free form at 2.0 A and 3.0 A resolution, respectively. Previous biochemical characterizations have demonstrated that eukaryotic enzymes behave as homodimers in contrast to prokaryotic HPPDs, which are homotetramers. Plant and bacterial enzymes share the overall fold but use orthogonal surfaces for oligomerization. In addition, comparison of both structures provides direct evidence that the C-terminal helix gates substrate access to the active site around a nonheme ferrous iron center. In the Z. mays HPPD structure this helix packs into the active site, sequestering it completely from the solvent. In contrast, in the Arabidopsis structure this helix tilted by about 60 degrees into the solvent and leaves the active site fully accessible. By elucidating the structure of plant HPPD enzymes we aim to provide a structural basis for the development of new herbicides.
+
-
 
+
-
==About this Structure==
+
-
1SP9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SP9 OCA].
+
-
 
+
-
==Reference==
+
-
The crystal structures of Zea mays and Arabidopsis 4-hydroxyphenylpyruvate dioxygenase., Fritze IM, Linden L, Freigang J, Auerbach G, Huber R, Steinbacher S, Plant Physiol. 2004 Apr;134(4):1388-400. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15084729 15084729]
+
-
[[Category: 4-hydroxyphenylpyruvate dioxygenase]]
+
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Auerbach, G.]]
+
[[Category: Auerbach G]]
-
[[Category: Freigang, J.]]
+
[[Category: Freigang J]]
-
[[Category: Fritze, I M.]]
+
[[Category: Fritze IM]]
-
[[Category: Huber, R.]]
+
[[Category: Huber R]]
-
[[Category: Linden, L.]]
+
[[Category: Linden L]]
-
[[Category: Steinbacher, S.]]
+
[[Category: Steinbacher S]]
-
[[Category: oxidoreductase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:45:14 2008''
+

Current revision

4-Hydroxyphenylpyruvate Dioxygenase

PDB ID 1sp9

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1sp9"
Personal tools