5n77
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5n77 is ON HOLD Authors: Description: Category: Unreleased Structures) |
|||
| (4 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the cytosolic domain of the CorA magnesium channel from Escherichia coli in complex with magnesium== | |
| + | <StructureSection load='5n77' size='340' side='right'caption='[[5n77]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5n77]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N77 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n77 OCA], [https://pdbe.org/5n77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n77 RCSB], [https://www.ebi.ac.uk/pdbsum/5n77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n77 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CORA_ECOLI CORA_ECOLI] Mediates influx of magnesium ions. Can also mediate cobalt and manganese uptake (PubMed:780341). Alternates between open and closed states. Activated by low cytoplasmic Mg(2+) levels. Inactive when cytoplasmic Mg(2+) levels are high (By similarity).[UniProtKB:Q9WZ31]<ref>PMID:780341</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Structures of the Mg2+ bound (closed) and apo (open) states of CorA suggests that channel gating is accomplished by rigid-body motions between symmetric and asymmetric assemblies of the cytosolic portions of the five subunits in response to ligand (Mg2+) binding/unbinding at interfacial sites. Here, we structurally and biochemically characterize the isolated cytosolic domain from Escherichia coli CorA. The data reveal an Mg2+-ligand binding site located in a novel position between each of the five subunits and two Mg2+ ions trapped inside the pore. Soaking experiments show that cobalt hexammine outcompetes Mg2+ at the pore site closest to the membrane. This represents the first structural information of how an analog of hexa-hydrated Mg2+ (and competitive inhibitor of CorA) associates to the CorA pore. Biochemical data on the isolated cytoplasmic domain and full-length protein suggests that gating of the CorA channel is governed cooperatively. | ||
| - | + | Structure and Cooperativity of the Cytosolic Domain of the CorA Mg2+ Channel from Escherichia coli.,Lerche M, Sandhu H, Flockner L, Hogbom M, Rapp M Structure. 2017 Aug 1;25(8):1175-1186.e4. doi: 10.1016/j.str.2017.05.024. Epub, 2017 Jun 29. PMID:28669631<ref>PMID:28669631</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5n77" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Escherichia coli]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Flockner L]] | ||
| + | [[Category: Hogbom M]] | ||
| + | [[Category: Lerche M]] | ||
| + | [[Category: Rapp M]] | ||
| + | [[Category: Sandhu H]] | ||
Current revision
Crystal structure of the cytosolic domain of the CorA magnesium channel from Escherichia coli in complex with magnesium
| |||||||||||
