5n8y
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5n8y is ON HOLD Authors: Schuller, J.M., Snijder, J., Loessl, P., Heck, A.J.R., Foerster, F. Description: KaiCBA circadian clock backbone model bas...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==KaiCBA circadian clock backbone model based on a Cryo-EM density== | |
| + | <SX load='5n8y' size='340' side='right' viewer='molstar' caption='[[5n8y]], [[Resolution|resolution]] 4.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5n8y]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus Synechococcus elongatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N8Y FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.7Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n8y OCA], [https://pdbe.org/5n8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n8y RCSB], [https://www.ebi.ac.uk/pdbsum/5n8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n8y ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/KAIC_SYNE7 KAIC_SYNE7] Core component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. Binds to DNA. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction.<ref>PMID:9727980</ref> <ref>PMID:14709675</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cyanobacteria have a robust circadian oscillator, known as the Kai system. Reconstituted from the purified protein components KaiC, KaiB, and KaiA, it can tick autonomously in the presence of adenosine 5'-triphosphate (ATP). The KaiC hexamers enter a natural 24-hour reaction cycle of autophosphorylation and assembly with KaiB and KaiA in numerous diverse forms. We describe the preparation of stoichiometrically well-defined assemblies of KaiCB and KaiCBA, as monitored by native mass spectrometry, allowing for a structural characterization by single-particle cryo-electron microscopy and mass spectrometry. Our data reveal details of the interactions between the Kai proteins and provide a structural basis to understand periodic assembly of the protein oscillator. | ||
| - | + | Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state.,Snijder J, Schuller JM, Wiegard A, Lossl P, Schmelling N, Axmann IM, Plitzko JM, Forster F, Heck AJ Science. 2017 Mar 17;355(6330):1181-1184. doi: 10.1126/science.aag3218. Epub 2017, Mar 16. PMID:28302852<ref>PMID:28302852</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5n8y" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: Loessl | + | *[[Circadian clock protein 3D structures|Circadian clock protein 3D structures]] |
| - | [[Category: Schuller | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synechococcus elongatus]] | ||
| + | [[Category: Foerster F]] | ||
| + | [[Category: Heck AJR]] | ||
| + | [[Category: Loessl P]] | ||
| + | [[Category: Schuller JM]] | ||
| + | [[Category: Snijder J]] | ||
Current revision
KaiCBA circadian clock backbone model based on a Cryo-EM density
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