This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1sr4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:34, 1 May 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1sr4.jpg|left|200px]]
 
-
{{Structure
+
==Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin==
-
|PDB= 1sr4 |SIZE=350|CAPTION= <scene name='initialview01'>1sr4</scene>, resolution 2.0&Aring;
+
<StructureSection load='1sr4' size='340' side='right'caption='[[1sr4]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>
+
<table><tr><td colspan='2'>[[1sr4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_ducreyi Haemophilus ducreyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SR4 FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
|GENE= CDTA, HD0902 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=730 Haemophilus ducreyi])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr4 OCA], [https://pdbe.org/1sr4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sr4 RCSB], [https://www.ebi.ac.uk/pdbsum/1sr4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sr4 ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr4 OCA], [http://www.ebi.ac.uk/pdbsum/1sr4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sr4 RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/CDTA_HAEDU CDTA_HAEDU] CDTs are cytotoxins which induce host cell distension, growth arrest in G2/M phase, nucleus swelling, and chromatin fragmentation in HeLa cells. CdtA, along with CdtC, probably forms a heterodimeric subunit required for the delivery of CdtB.
-
 
+
== Evolutionary Conservation ==
-
'''Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin'''
+
[[Image:Consurf_key_small.gif|200px|right]]
-
 
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==Overview==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sr/1sr4_consurf.spt"</scriptWhenChecked>
-
The tripartite cytolethal distending toxin (CDT) induces cell cycle arrest and apoptosis in eukaryotic cells. The subunits CdtA and CdtC associate with the nuclease CdtB to form a holotoxin that translocates CdtB into the host cell, where it acts as a genotoxin by creating DNA lesions. Here we show that the crystal structure of the holotoxin from Haemophilus ducreyi reveals that CDT consists of an enzyme of the DNase-I family, bound to two ricin-like lectin domains. CdtA, CdtB and CdtC form a ternary complex with three interdependent molecular interfaces, characterized by globular, as well as extensive non-globular, interactions. The lectin subunits form a deeply grooved, highly aromatic surface that we show to be critical for toxicity. The holotoxin possesses a steric block of the CdtB active site by means of a non-globular extension of the CdtC subunit, and we identify putative DNA binding residues in CdtB that are essential for toxin activity.
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==About this Structure==
+
</jmolCheckbox>
-
1SR4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Haemophilus_ducreyi Haemophilus ducreyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA].
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sr4 ConSurf].
-
 
+
<div style="clear:both"></div>
-
==Reference==
+
__TOC__
-
Assembly and function of a bacterial genotoxin., Nesic D, Hsu Y, Stebbins CE, Nature. 2004 May 27;429(6990):429-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15164065 15164065]
+
</StructureSection>
-
[[Category: Haemophilus ducreyi]]
+
[[Category: Large Structures]]
-
[[Category: Protein complex]]
+
[[Category: Hsu Y]]
-
[[Category: Hsu, Y.]]
+
[[Category: Nesic D]]
-
[[Category: Nesic, D.]]
+
[[Category: Stebbins CE]]
-
[[Category: Stebbins, C E.]]
+
-
[[Category: apoptosis]]
+
-
[[Category: bacterial]]
+
-
[[Category: cell cycle]]
+
-
[[Category: cytotoxin]]
+
-
[[Category: deoxyribonuclease i]]
+
-
[[Category: dna damage]]
+
-
[[Category: genotoxin]]
+
-
[[Category: haemophilus ducreyi]]
+
-
[[Category: lectin]]
+
-
[[Category: toxin]]
+
-
[[Category: virulence]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:45:57 2008''
+

Current revision

Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin

PDB ID 1sr4

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1sr4"
Personal tools